BDE 209 对人胎盘来源的 JEG 细胞中胎盘脱碘酶 3 表达的改变及牛磺酸的可能保护作用在低氧条件下。

Alteration of Placental Deiodinase 3 Expression by BDE 209 and Possible Protection by Taurine in Human Placenta-Derived JEG Cells Under Hypoxia.

机构信息

Dalian Key Laboratory of Hematology; Department of Occupational and Environmental Health, School of Public Health, Dalian Medical University, Dalian, Liaoning, China.

Department of Occupational and Environmental Health, School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.

出版信息

Adv Exp Med Biol. 2019;1155:775-785. doi: 10.1007/978-981-13-8023-5_67.

DOI:10.1007/978-981-13-8023-5_67

PMID:31468447

Abstract

Thyroid hormones are key hormones involved in growth and development. Changes in their levels can cause embryonic brain developmental damage in the first trimester. Studies have shown that polybrominated diphenyl ethers (PBDEs) have developmental neurotoxicity as environmental pollutants, and exposure during pregnancy can cause irreversible brain damage in offspring, similar to the interference effects of thyroid hormones, but its mechanism has not yet been understood. Since the physiological environment for placental cells is highly hypoxic, in the current study, the human placenta-derived JEG cells were cultured at 1% oxygen, 4% carbon dioxide and 94% nitrogen, to reflect in vivo scenario, and the possible protection of taurine on BDE 209-mediated toxicity in JEG cells was studied. Our data showed that different concentrations of BDE 209 can have profound effects on cell viability and placental deiodinase 3 expression under hypoxic culture condition. Taurine was found to improve BDE 209-induced reductions in cell viability and altered gene and protein expressions of placental deiodinases. The results provide a reference for the establishment of early biomarkers and effective preventive measures.

摘要

甲状腺激素是参与生长和发育的关键激素。其水平的变化会导致妊娠早期胚胎大脑发育受损。研究表明，多溴二苯醚（PBDEs）作为环境污染物具有发育神经毒性，妊娠期间暴露会导致后代不可逆转的脑损伤，类似于甲状腺激素的干扰作用，但具体机制尚不清楚。由于胎盘细胞的生理环境高度缺氧，在本研究中，用人胎盘来源的 JEG 细胞在 1%氧气、4%二氧化碳和 94%氮气中进行培养，以模拟体内情况，并研究牛磺酸对 JEG 细胞中 BDE 209 介导的毒性的可能保护作用。我们的数据表明，不同浓度的 BDE 209 在低氧培养条件下对细胞活力和胎盘脱碘酶 3 的表达有显著影响。牛磺酸可改善 BDE 209 引起的细胞活力降低以及胎盘脱碘酶基因和蛋白表达的改变。研究结果为建立早期生物标志物和有效预防措施提供了参考。