通过测序研究牛磺酸处理后糖尿病神经病变大鼠坐骨神经中的 microRNAs 表达谱。

The microRNAs Expression Profile in Sciatic Nerves of Diabetic Neuropathy Rats After Taurine Treatment by Sequencing.

机构信息

Department of Occupational and Environmental Health, Dalian Medical University, Dalian, China.

Laboratory Animal Center, Dalian Medical University, Dalian, China.

出版信息

Adv Exp Med Biol. 2019;1155:935-947. doi: 10.1007/978-981-13-8023-5_78.

DOI:10.1007/978-981-13-8023-5_78

PMID:31468458

Abstract

Taurine protect against diabetic neuropathy. However, the protective mechanism of taurine has been poorly understood. It has been demonstrated that microRNAs (miRNAs) are involved in regulating gene expression. Therefore, it is interested in whether taurine affects miRNAs expression profile in peripheral nerve tissue of diabetic neuropathy. In the present study, rats were treated as three group: (1) control (Con) group, (2) diabetic mellitus (DM) group and (3) taurine treatment (Tau) group. Sciatic nerve tissue was harvested and miRNA expression was determined using sequencing. The results showed that 80 miRNAs showed significant difference in DM group compared to Con group, of which 20 miRNAs showed up-regulating, as well as, 60 miRNAs showed down-regulating. On the other hand, 215 differential miRNAs were found between DM and Tau groups. Moreover, the numbers of up-regulated and down-regulated miRNAs were 1 and 214, respectively. Twelve specific miRNAs were screened out and the target genes were obtained by target analysis software. GO and KEGG enrichment analyses showed that these potential target genes for the miRNAs might be involved in axon guidance, generation of neurons, nervous system development and neurogenesis. Our results provided a miRNA profile for further exploring protective mechanisms of taurine against diabetic peripheral neuropathy.

摘要

牛磺酸可预防糖尿病性周围神经病。然而，牛磺酸的保护机制还未被充分了解。已经证明，microRNAs (miRNAs) 参与调节基因表达。因此，人们对牛磺酸是否影响糖尿病性周围神经病患者周围神经组织中的 miRNA 表达谱很感兴趣。在本研究中，大鼠分为三组：（1）对照组（Con），（2）糖尿病组（DM）和（3）牛磺酸处理组（Tau）。采集坐骨神经组织，使用测序法测定 miRNA 表达。结果显示，与 Con 组相比，DM 组有 80 个 miRNA 表达显著差异，其中 20 个 miRNA 上调，60 个 miRNA 下调。另一方面，在 DM 和 Tau 组之间发现了 215 个差异 miRNA。此外，上调和下调 miRNA 的数量分别为 1 和 214。筛选出 12 个特定 miRNA，并通过靶标分析软件获得靶基因。GO 和 KEGG 富集分析表明，这些 miRNA 的潜在靶基因可能参与轴突导向、神经元生成、神经系统发育和神经发生。我们的研究结果为进一步探索牛磺酸预防糖尿病性周围神经病的保护机制提供了 miRNA 图谱。