p53 免疫染色模式为野生型的卵巢输卵管腹膜高级别浆液性癌的靶向测序。
Targeted Sequencing of Tubo-ovarian and Peritoneal High-grade Serous Carcinoma With Wild-type p53 Immunostaining Pattern.
机构信息
Department of Pathology and Translational Genomics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
Department of Pathology, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.
出版信息
In Vivo. 2019 Sep-Oct;33(5):1485-1492. doi: 10.21873/invivo.11628.
Abstract
BACKGROUND/AIM: We aimed to demonstrate the use of next-generation sequencing (NGS) to confirm the presence of tumor protein 53 (TP53) mutations in tubo-ovarian and peritoneal high-grade serous carcinoma (HGSC) with a wild-type p53 immunostaining pattern and investigate whether the TP53 mutational status is altered by chemotherapy.
MATERIALS AND METHODS
A commercial NGS panel comprising 171 genes was used to analyze the genetic profiles of 15 HGSC samples. Paired specimens obtained before and after chemotherapy were available for four patients.
RESULTS
All examined samples exhibited TP53 mutations. For all the patients who underwent neoadjuvant or postoperative adjuvant chemotherapy, TP53 mutations identified in samples obtained after chemotherapy were the same as those detected in pre-chemotherapeutic samples.
CONCLUSION
HGSCs exhibit TP53 mutations even though a subset of HGSCs displayed a wild-type p53 immunostaining pattern. Chemotherapy does not affect the TP53 mutational status in HGSC.
摘要
背景/目的：我们旨在展示下一代测序（NGS）在伴有野生型 p53 免疫组化模式的卵巢输卵管和腹膜高级别浆液性癌（HGSC）中用于证实肿瘤蛋白 53（TP53）突变的存在，并研究化疗是否会改变 TP53 突变状态。
材料和方法
使用包含 171 个基因的商业 NGS 面板分析了 15 例 HGSC 样本的遗传特征。四个患者的配对标本在化疗前后均可用。
结果
所有检查的样本均显示 TP53 突变。对于所有接受新辅助或术后辅助化疗的患者，化疗后获得的样本中检测到的 TP53 突变与化疗前样本中检测到的突变相同。
结论
即使一部分 HGSCs 显示野生型 p53 免疫组化模式，HGSCs 仍显示 TP53 突变。化疗不会影响 HGSC 中的 TP53 突变状态。
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