Semancik G J, Shust N M, Byrnes S A, Nishioka D
Department of Biology, Georgetown University, Washington, D.C. 20057.
Cell Biol Int Rep. 1988 Oct;12(10):857-66. doi: 10.1016/0309-1651(88)90049-5.
The cortical reaction in isolated sea urchin (Strongylocentrotus purpuratus) egg cortices has been monitored with phase-contrast video microscopy. It was confirmed that the cortical reaction is induced by exposure to Ca2+. No induction was observed after exposure to the Ca2+-ionophore A23187, although the cortices remain sensitive to a subsequent exposure to Ca2+, and the cortical reaction in unfertilized eggs suspended in cortex isolation medium remains inducible by exposure to A23187. These results imply: (1) that A23187 does not induce the cortical reaction directly; (2) that the release of intracellular Ca2+, through which A23187 induces the cortical reaction, is not from storage sites localized entirely in the cortex; and (3) that intracellular storage sites for the Ca2+ involved in the cortical reaction are also present outside the cortex.
利用相差视频显微镜监测了分离的海胆（紫海胆）卵皮质中的皮质反应。已证实皮质反应是由暴露于Ca2+诱导的。暴露于Ca2+离子载体A23187后未观察到诱导作用，尽管皮质对随后暴露于Ca2+仍保持敏感，并且悬浮在皮质分离培养基中的未受精卵中的皮质反应在暴露于A23187后仍可诱导。这些结果表明：（1）A23187不直接诱导皮质反应；（2）A23187诱导皮质反应所通过的细胞内Ca2+释放并非完全来自定位于皮质中的储存位点；（3）参与皮质反应的Ca2+的细胞内储存位点也存在于皮质之外。
