Marsupials: Progress and Prospects.

The chapter provides a review of the application of reproductive science to technologies for marsupial conservation and population management and discusses prospects for the future. This includes the status of technologies such as sperm freezing, artificial insemination, and exogenous hormone treatments to stimulate ovarian activity and cycling in the female. Fertility-based population management for introduced pest species and over-abundant native marsupials remain an elusive goal. Immune-contraceptive approaches, despite demonstration of basic effectiveness, have not progressed to field deliverable agents. Emerging genetic technologies such as gene drives offer great promise, but gene modifications face major challenges to be broadly accepted both socially and politically. A main theme is the potential advantages, both genetic and economic, of integrating frozen stored genomic material, such as sperm, into the captive breeding component of threatened species strategies. However, the sperm of many marsupial species display no or very poor recovery of motility on thawing. For this reason, it is proposed that the traditional assisted breeding paradigm for conservation-cervical artificial insemination with thawed frozen sperm, based on cattle breeding-is not a viable default strategy. Rather, techniques such as sperm injection and emerging stem cell technologies that utilize stored frozen cells, and in the case of sperm, immotile cells, are better candidates for the development of a more generic approach. In addition, this change in focus encourages wide scale proactive genome storage when genetic diversity is greatest, without the need to demonstrate success in traditional sperm cryopreservation and thawing. However, the promise of the potential of reproductive science to conservation and non-lethal population management is problematic without far greater recognition of, and investment in, the needs of wildlife by society.