Department of Obstetrics, Gynecology and Reproductive Sciences, Division of Reproductive Sciences, Yale School of Medicine, New Haven, CT, USA.
Department of Obstetrics, Gynecology and Reproductive Sciences, Division of Reproductive Sciences, Yale School of Medicine, New Haven, CT, USA; Department of Obstetrics and Gynecology, University of Helsinki and the Helsinki University Hospital, Finland.
Cytokine. 2020 Jan;125:154829. doi: 10.1016/j.cyto.2019.154829. Epub 2019 Aug 28.
The role of cytokines in various disease states is a burgeoning field of academic study and clinical application, however there are no consensus documents on how certain cytokines should be stored prior to quantification. This information is especially of interest to researchers assembling a biobank or clinicians who have to transport specimens to a different location in order to be tested.
To review the literature and synthesize prior findings on cytokine storage and freeze/thaw stability.
We searched PubMed for articles related to cytokine storage stability. All articles were analyzed for cytokines studied, source of reported cytokine concentration (i.e., human whole blood or serum, concentrations from other species or bodily sources were excluded), and reported statistical results.
We identified and synthesized results of 23 peer-reviewed articles which published data on the storage and freeze/thaw stability of 33 different cytokines and chemokines.
There is a wide variety of reported cytokine storage and freeze/thaw stability. Interleukin-6 and tumor necrosis factor alpha are the most widely studied cytokines in regard to temperature stability. In a few cytokines, a clear consensus can be reached as to storage safety at particular temperatures, but in most, more research needs to be done and we advise the clinician or researcher to use caution in interpreting cytokine concentration results after a long period of storage or several freeze/thaw cycles.
细胞因子在各种疾病状态中的作用是学术研究和临床应用中一个新兴的领域，然而，对于某些细胞因子在定量分析之前应该如何储存，目前还没有共识文件。对于正在组建生物库的研究人员或需要将标本运输到不同地点进行检测的临床医生来说，这些信息尤为重要。
回顾文献并综合关于细胞因子储存和冻融稳定性的现有发现。
我们在 PubMed 上搜索了与细胞因子储存稳定性相关的文章。对所有文章进行了分析，以确定研究的细胞因子、报告细胞因子浓度的来源（即人全血或血清，排除来自其他物种或其他身体来源的浓度）以及报告的统计结果。
我们确定并综合了 23 篇同行评审文章的结果，这些文章发表了关于 33 种不同细胞因子和趋化因子的储存和冻融稳定性的数据。
报告的细胞因子储存和冻融稳定性差异很大。白细胞介素 6 和肿瘤坏死因子-α是关于温度稳定性研究最多的细胞因子。对于某些细胞因子，可以就特定温度下的储存安全性达成明确共识，但对于大多数细胞因子，还需要进行更多的研究，我们建议临床医生或研究人员在储存时间过长或经过多次冻融循环后，谨慎解释细胞因子浓度结果。
