Institute of Biochemistry and Genetics, Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, pr. Oktyabrya 71, 450054, Ufa, Russia; Bashkir State University, Z. Validi str. 32, 450074, Ufa, Russia.
Institute of Biochemistry and Genetics, Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, pr. Oktyabrya 71, 450054, Ufa, Russia.
J Plant Physiol. 2019 Nov;242:153033. doi: 10.1016/j.jplph.2019.153033. Epub 2019 Aug 22.
A small family of ARGOS genes encodes transmembrane proteins that act as negative regulators of ethylene signaling. Recent studies show that ARGOS genes are involved in the regulation of plant growth under the influence of stress factors. However, the role of ARGOS genes in this process is poorly known. Thereby, our goal was to determine the expression profile of these genes in Arabidopsis thaliana and Nicotiana tabacum in response to phytohormone treatment and stress factors. We discovered that expression of the AtARGOS and AtARGOS-LIKE genes of A. thaliana is regulated by ethylene and depends on environmental conditions. The highest expression level of the NtARGOS-LIKE1 gene of tobacco (NtARL1) was observed in blooming flowers and young organs. It was induced by auxins, ethylene, ABA, methyl jasmonate as well as hypothermia, drought, salinity and heat stresses. To evaluate the impact of ARGOS genes on plant growth under stress, we created transgenic tobacco plants with constitutive expression of the AtARGOS-LIKE gene of A. thaliana (AtARL), controlled by a strong Dahlia mosaic virus promoter. Overexpression of the AtARL gene contributed to an increase in the volume and quantity of mesophyll cells in the leaves of tobacco under normal conditions, and also to an improvement in root growth under salinity, cold and cadmium treatment. The AtARL transgene produced a positive effect on shoot growth when exposed to drought and high salinity, and a negative effect under cold stress. Accordingly, genes of the ARGOS family can be recommended as targets for genetic engineering and genome editing in order to enhance productivity and stress tolerance of economically important plants.
一个小的 ARGOS 基因家族编码跨膜蛋白，作为乙烯信号的负调节剂。最近的研究表明，ARGOS 基因参与了植物在胁迫因子影响下的生长调节。然而，ARGOS 基因在这一过程中的作用知之甚少。因此，我们的目标是确定拟南芥和烟草中这些基因在植物激素处理和胁迫因子下的表达谱。我们发现，拟南芥的 AtARGOS 和 AtARGOS-LIKE 基因的表达受乙烯调控，并依赖于环境条件。烟草（NtARL1）的 NtARGOS-LIKE1 基因的表达水平最高，在开花期和幼嫩器官中观察到。它被生长素、乙烯、ABA、茉莉酸甲酯以及低温、干旱、盐和热胁迫诱导。为了评估 ARGOS 基因在胁迫下对植物生长的影响，我们通过组成型表达拟南芥的 AtARGOS-LIKE 基因（AtARL），利用强的大丽花 mosaic 病毒启动子，创建了转基因烟草植株。在正常条件下，AtARL 基因的过表达导致烟草叶片中叶肉细胞的体积和数量增加，同时在盐、冷和镉处理下也改善了根的生长。在干旱和高盐胁迫下，AtARL 转基因对芽生长有积极影响，而在冷胁迫下则有负面影响。因此，ARGOS 家族的基因可以作为遗传工程和基因组编辑的靶点，以提高经济上重要的植物的生产力和抗逆性。
