Berntsen P, Hadian Jazi M, Kusel M, Martin A V, Ericsson T, Call M J, Trenker R, Roque F G, Darmanin C, Abbey B
Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3086, Australia.
Kusel Design, 12 Coghlan Street, Niddrie, VIC 3042, Australia.
Rev Sci Instrum. 2019 Aug;90(8):085110. doi: 10.1063/1.5104298.
A serial millisecond crystallography (SMX) facility has recently been implemented at the macromolecular crystallography beamline, MX2 at the Australian Synchrotron. The setup utilizes a combination of an EIGER X 16M detector system and an in-house developed high-viscosity injector, "Lipidico." Lipidico uses a syringe needle to extrude the microcrystal-containing viscous media and it is compatible with commercially available syringes. The combination of sample delivery via protein crystals suspended in a viscous mixture and a millisecond frame rate detector enables high-throughput serial crystallography at the Australian Synchrotron. A hit-finding algorithm, based on the principles of "robust-statistics," is employed to rapidly process the data. Here we present the first SMX experimental results with a detector frame rate of 100 Hz (10 ms exposures) and the Lipidico injector using a mixture of lysozyme microcrystals embedded in high vacuum silicon grease. Details of the experimental setup, sample injector, and data analysis pipeline are designed and developed as part of the Australian Synchrotron SMX instrument and are reviewed here.
澳大利亚同步加速器的大分子晶体学光束线MX2最近启用了串行毫秒晶体学（SMX）设备。该装置采用了EIGER X 16M探测器系统和内部开发的高粘度注射器“Lipidico”相结合的方式。Lipidico使用注射器针头挤出含微晶的粘性介质，并且与市售注射器兼容。通过悬浮在粘性混合物中的蛋白质晶体进行样品输送，再结合毫秒帧率探测器，使得澳大利亚同步加速器能够进行高通量串行晶体学研究。一种基于“稳健统计”原理的命中发现算法被用于快速处理数据。在此，我们展示了使用Lipidico注射器、探测器帧率为100 Hz（10毫秒曝光），以嵌入高真空硅脂中的溶菌酶微晶混合物进行的首次SMX实验结果。实验装置、样品注射器和数据分析流程的详细信息是作为澳大利亚同步加速器SMX仪器的一部分设计和开发的，在此进行介绍。
