Department of Chemistry, University of the Balearic Islands, Cra.Valldemossa Km 7.5, 07122, Palma de Mallorca, Spain.
Sciware Systems, S.L., Spin-off of the University of the Balearic Islands, C/Pi 37, 07193, Bunyola, Spain.
Anal Chim Acta. 2019 Nov 15;1082:56-65. doi: 10.1016/j.aca.2019.06.032. Epub 2019 Jun 19.
An on-line lab-on-valve system for renewable micro-solid phase extraction coupled with high performance liquid chromatography with photodiode array detection was developed for the determination of hesperidin and diosmin in natural and commercial citrus samples. The method is based on the automatic loading of 8 mL of sample into the C resin, followed by matrix clean-up and elution of the analytes with 0.27 mL of acetonitrile. Next, 0.02 mL of the extract were injected into the chromatographic system. Separation was performed by using a Symmetry C column (250 × 3.0 mm x 5.0 μm) with a mobile phase consisting in a mixture of methanol, acetonitrile and acidic water (pH = 2.5) in gradient mode at 0.58 mL min. The detection wavelength was chosen as 285 nm. Limits of detection, quantification and relative standard deviations were lower than 0.1 μg mL, 0.3 μg mL and 4.1%, respectively.
建立了一种在线阀上实验室系统，用于可再生微固相萃取，结合高效液相色谱与光电二极管阵列检测，用于测定天然和商业柑橘样品中的橙皮苷和橙皮苷。该方法基于自动加载 8mL 样品到 C 树脂上，然后用 0.27mL 乙腈进行基质净化和分析物洗脱。接下来，将 0.02mL 提取物注入色谱系统。采用甲醇、乙腈和酸性水（pH=2.5）的混合物作为流动相，在 0.58mL min 的梯度模式下，在 Symmetry C 柱（250×3.0mm x 5.0μm）上进行分离。检测波长选为 285nm。检测限、定量限和相对标准偏差分别低于 0.1μg/mL、0.3μg/mL 和 4.1%。
