Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43110, USA.
Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43110, USA.
Anal Chim Acta. 2019 Nov 15;1082:98-105. doi: 10.1016/j.aca.2019.07.015. Epub 2019 Jul 9.
Storage and quantitative analysis of small volumes of biofluids are challenging, especially when low concentrations of analytes are to be detected in the presence of complex matrices. In this study, we describe an integrated thread-based approach for stabilizing small blood volumes in the dry-state at room temperature, while also offering direct analysis capabilities via thread spray mass spectrometry. The analytical merits of this novel microsampling platform was demonstrated via the direct analysis of diazepam and cocaine in dried blood samples stored for 42 days. In-situ in-capillary blood processing from hydrophobic threads enabled limits of detection as low as parts-per-quadrillion to be reached. We validated this ultra-sensitivity by analyzing small tissue-like residues collected after pushing a thread through the sample once. The implications of this sample collection, storage, and analysis platform can be extensive with direct applications in forensics and clinical studies.
储存和定量分析小体积的生物流体具有挑战性，特别是在存在复杂基质的情况下，需要检测低浓度的分析物。在本研究中，我们描述了一种集成的基于线的方法，用于在室温下将小体积的血液稳定在干燥状态，同时通过线喷雾质谱提供直接分析能力。通过直接分析在干燥血液样本中储存 42 天的地西泮和可卡因，证明了这种新型微采样平台的分析优点。从疏水管线中进行的原位在管内血液处理能够达到低至万亿分之几的检测限。我们通过分析通过样本推动线一次后收集的小组织状残留物来验证这种超灵敏度。这种样品收集、储存和分析平台的意义可能非常广泛，直接应用于法医学和临床研究。
