Department of Chemistry and Biochemistry , The Ohio State University , Columbus , Ohio 43210 , United States.
Department of Materials Science and Engineering , Iowa State University , Ames , Iowa 50011 , United States.
Anal Chem. 2018 Aug 7;90(15):9353-9358. doi: 10.1021/acs.analchem.8b01962. Epub 2018 Jul 18.
It is well-known that 2D dried blood spots on paper offer a facile sample collection, storage, and transportation of blood. However, large volume requirements, possible analyte instability, and difficult sample recovery plague this method, lowering confidence in analyte quantification. For the first time, we demonstrate a new approach using 3D dried blood spheroids for stabilization of small volume blood samples, mitigating these effects without cold storage. Blood spheroids form on hydrophobic paper, preventing interaction between the sample and paper substrate, eliminating all chromatographic effects. Stability of the enzyme alanine transaminase and labile organic compounds such as cocaine and diazepam were also shown to increase in the spheroid by providing a critical radius of insulation. On-surface analysis of the dried blood spheroids using paper spray mass spectrometry resulted in sub-ng/mL limits of detection for all illicit drugs tested, representing 1 order of magnitude improvement compared with analysis from 2D dried blood spots.
众所周知，纸质二维干血斑可轻松采集、储存和运输血液样本。然而，这种方法存在样本体积需求大、分析物可能不稳定以及难以回收样本等问题，降低了对分析物定量的信心。本文首次提出了一种新方法，使用三维干血球来稳定小体积血液样本，在不进行冷藏的情况下减轻这些影响。血球在疏水纸上形成，防止了样本与纸基质之间的相互作用，消除了所有的色谱效应。研究还表明，通过提供临界绝缘半径，酶丙氨酸转氨酶和不稳定的有机化合物（如可卡因和地西泮）的稳定性也得到了提高。使用纸喷雾质谱对干燥血球进行表面分析，检测到所有检测的非法药物的检出限均达到纳克/毫升级，与二维干血斑分析相比，提高了 1 个数量级。
