Maia Carla, Cristóvão José Manuel, Pereira André, Parreira Ricardo, Campino Lenea
Global Health and Tropical Medicine (GHTM), Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa (UNL), Lisbon, Portugal; Medical Parasitology Unit, IHMT-UNL, Lisbon, Portugal.
Global Health and Tropical Medicine (GHTM), Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa (UNL), Lisbon, Portugal; Medical Parasitology Unit, IHMT-UNL, Lisbon, Portugal.
Top Companion Anim Med. 2019 Sep;36:12-15. doi: 10.1053/j.tcam.2019.06.001. Epub 2019 Jun 25.
Vector-borne rickettsioses represent emerging threats to public health worldwide. The aim of this work was the screening for the presence of Rickettsia spp. in the blood of dogs and cats from southern Portugal. A PCR product of the expected size was amplified from DNA extracts obtained from blood samples of 29 out of 225 (12.9%) cats and in 2 out of 375 (.5%) dogs using genus-specific primers targeting Rickettsia gltA. Rickettsia conorii israelensis was identified by phylogenetical analysis of partial ompB sequences, amplified from blood samples taken from both a cat and a dog. The obtained results reinforce the idea that domestic animals may act as sentinels for the presence of vector-borne Rickettsia spp. in a given geographical area. In addition, rickettsioses should be included in the differential diagnosis of canine and feline vector-borne diseases.
媒介传播的立克次氏体病对全球公共卫生构成新的威胁。这项工作的目的是筛查葡萄牙南部猫狗血液中是否存在立克次氏体属。使用针对立克次氏体属柠檬酸合酶基因（gltA）的属特异性引物，从225只猫中的29只（12.9%）和375只狗中的2只（0.5%）的血液样本DNA提取物中扩增出预期大小的PCR产物。通过对从一只猫和一只狗采集的血液样本中扩增的部分外膜蛋白B（ompB）序列进行系统发育分析，鉴定出以色列康氏立克次氏体。所得结果强化了这样一种观点，即家畜可能作为特定地理区域内媒介传播立克次氏体属存在的哨兵。此外，立克次氏体病应纳入犬猫媒介传播疾病的鉴别诊断中。
