一种用于抗人绒毛膜促性腺激素疫苗的改良免疫原，可引发与该激素天然构象反应的抗体，且不与人促卵泡激素和人促甲状腺激素发生交叉反应。

An improved immunogen for anti-human chorionic gonadotropin vaccine eliciting antibodies reactive with a conformation native to the hormone without cross-reaction with human follicle stimulating hormone and human thyroid stimulating hormone.

作者信息

Talwar G P, Singh O, Rao L V

机构信息

Immuno-Endocrinology Group, National Institute of Immunology, New Delhi, India.

出版信息

J Reprod Immunol. 1988 Dec;14(3):203-12. doi: 10.1016/0165-0378(88)90020-4.

DOI:10.1016/0165-0378(88)90020-4

PMID:3147333

Abstract

Beta-subunit of human chorionic gonadotropin (hCG) was associated with alpha-subunit of ovine luteinizing hormone (OLH) to create a heterospecies dimer (HSD) which has a higher steroidogenic potency than the homologous dimer of alpha hCG and beta hCG in the mouse Leydig cell bioassay. The properties and merits of the antibodies induced by this HSD and beta hCG linked to carrier(s) were investigated in rodents and in a subhuman primate species. The antisera had, in both cases, high affinity for binding with hCG (K alpha = 10(9)-10(10) M-1). The mean (+/- S.E.M.) bioneutralization capacity as a percentage of immunoreactivity (determined by radioimmunoassay (RIA)) of the antibodies generated by the HSD-carrier in rats and bonnet monkeys was higher in comparison with those induced by beta hCG linked to carrier (80 +/- 2.3% vs. 63 +/- 1.5% in rats, and 65 +/- 1.9% for HSD vs. 44 +/- 3.7% in monkeys). None of the sera gave any evidence of cross-reactivity with human follicle stimulating hormone (hFSH) and human thyroid stimulating hormone (hTSH).

摘要

人绒毛膜促性腺激素（hCG）的β亚基与绵羊促黄体生成素（OLH）的α亚基结合，形成一种异种二聚体（HSD），在小鼠睾丸间质细胞生物测定中，该异种二聚体比α-hCG和β-hCG的同源二聚体具有更高的类固醇生成能力。在啮齿动物和一种非人灵长类动物中研究了由这种HSD和与载体连接的β-hCG诱导的抗体的特性和优点。在这两种情况下，抗血清与hCG结合都具有高亲和力（Kα = 10⁹ - 10¹⁰ M⁻¹）。与由与载体连接的β-hCG诱导的抗体相比，HSD-载体在大鼠和冠毛猴中产生的抗体的生物中和能力平均（±标准误）占免疫反应性（通过放射免疫分析（RIA）测定）的百分比更高（大鼠中分别为80 ± 2.3% 对 63 ± 1.5%，猴子中HSD为65 ± 1.9% 对 44 ± 3.7%）。没有一份血清显示出与人类促卵泡激素（hFSH）和人类促甲状腺激素（hTSH）有交叉反应的迹象。