Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Department of Chemistry, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province, 130024, China.
Institute of Chemical and Industrial Bioengineering, Jilin Engineering Normal University, Changchun, Jilin Province, 130052, China.
J Chromatogr A. 2019 Oct 25;1604:460469. doi: 10.1016/j.chroma.2019.460469. Epub 2019 Aug 22.
While they are commonly used as ultraviolet (UV) filters in plastic food packaging materials, benzophenones (BPs) are reported as environmental endocrine disruptors, and some of them possess significant estrogenic activity. Therefore sensitive determination of the content of those UV filters in plastic polymers is of vital importance in safety assessment of food packaging materials. Here, the sheathless capillary electrophoresis-electrospray ionization-tandem mass spectrometry (CE-ESI-MS/MS) method is applied for the first time to sensitively detect BP-type UV filters in plastic food packaging materials. We investigate and optimize a variety of factors that may affect ESI-MS efficiency and CE separation. Sensitive detection of six BP-type UV filters is achieved using sheathless CE-ESI-MS/MS in conjunction with accelerated solvent extraction and solid phase extraction, with the limit-of-detection of 7 pg/mL-2.4 ng/mL. The method exhibits excellent inter/intra-day repeatability along with the advantages of efficient separation, rapid analysis, low sample consumption and high sensitivity. Six BP-type UV filters in eight different brands of plastic films obtained from supermarkets are successfully analyzed using the method. Good recoveries of 81.3-104.1% at three levels of spiked concentrations are achieved with low RSDs (n = 5) of 2.5-8.7%. Our study shows that the sheathless CE-ESI-MS/MS is a robust and reliable method for sensitive and rapid analysis of UV filters, which would be of potential application in safety assessment of plastic food packaging materials.
虽然苯并三唑类（BPs）通常被用作塑料食品包装材料中的紫外线（UV）过滤器，但它们被报道为环境内分泌干扰物，其中一些具有显著的雌激素活性。因此，敏感地测定塑料聚合物中这些 UV 过滤器的含量对于食品包装材料的安全评估至关重要。在这里，首次应用无鞘毛细管电泳-电喷雾电离-串联质谱（CE-ESI-MS/MS）方法灵敏地检测塑料食品包装材料中的 BP 型 UV 过滤器。我们研究并优化了多种可能影响 ESI-MS 效率和 CE 分离的因素。使用无鞘 CE-ESI-MS/MS 结合加速溶剂萃取和固相萃取，可实现对六种 BP 型 UV 过滤器的灵敏检测，检出限为 7 pg/mL-2.4 ng/mL。该方法具有高效分离、快速分析、低样品消耗和高灵敏度的优点，具有出色的日间/日内重复性。使用该方法成功分析了超市中 8 种不同品牌的塑料薄膜中的六种 BP 型 UV 过滤器。在三个加标浓度水平下，回收率为 81.3-104.1%，相对标准偏差（n=5）为 2.5-8.7%。我们的研究表明，无鞘 CE-ESI-MS/MS 是一种灵敏、快速分析 UV 过滤器的强大可靠方法，有望应用于塑料食品包装材料的安全评估。
