Sakamoto Miho, Minowa Keiko, Kishimoto Kiyoko, Nakajima Jun'ichi, Suzuki Jin, Moriyasu Takako, Fukaya Haruhiko, Saito Koichi
Tokyo Metropolitan Institute of Public Health.
Tokyo University of Pharmacy and Life Sciences.
Shokuhin Eiseigaku Zasshi. 2019;60(4):96-107. doi: 10.3358/shokueishi.60.96.
LC/Tribrid Orbitrap was developed to determine phosphodiesterase-5 (PDE-5) inhibitors and their analogs as adulterants in dietary supplements. High-resolution MS/MS and MS spectra of PDE-5 inhibitors and their analogs were obtained by LC/Tribrid Orbitrap using both higher-energy collisional dissociation and collision-induced dissociation. We investigated dietary supplements that claim to enhance men's sexual performance, and detected PDE-5 inhibitors and their analogs. We also estimated the structures of the PDE-5 inhibitor analogs and the impurities of PDE-5 inhibitors and their analogs in the dietary supplements.
液相色谱/三合一轨道阱质谱仪的开发是为了测定磷酸二酯酶-5（PDE-5）抑制剂及其类似物作为膳食补充剂中的掺假物。通过液相色谱/三合一轨道阱质谱仪，利用高能碰撞解离和碰撞诱导解离获得了PDE-5抑制剂及其类似物的高分辨率二级质谱和一级质谱。我们对声称能增强男性性功能的膳食补充剂进行了调查，并检测到了PDE-5抑制剂及其类似物。我们还对膳食补充剂中PDE-5抑制剂类似物的结构以及PDE-5抑制剂及其类似物的杂质进行了评估。
