Bayrak Omer, Basmaci Ismail, Bozdag Zehra, Sen Haluk, Erturhan Sakip, Balat Ayse, Seckiner Ilker
University of Gaziantep. School of Medicine. Gaziantep. Turkey.
Arch Esp Urol. 2019 Sep;72(7):690-696.
To compare c-kit-positive interstitial Cajal-like cells (ICC) and Caveolin-1 protein levels as a pacemaker and signaling molecules, on ureteropelvic junction (UPJ) specimens, between two groups of pediatric patients with and without ureteropelvic junction obstruction (UPJO).
We evaluated the UPJ specimens of 45 pediatric patients operated between 2005- 2012 retrospectively. Group 1 included 37 patients who underwent dismembered pyeloplasty due to UPJO. Eight patients underwent nephrectomy by the other reasons (renal tumor, trauma etc) and had normal UPJ were accepted as Group 2. The specimens were examined immunohistochemically with CD117 and Caveolin-1 antibody. According to the total number of ICC; 0-5 cells were accepted as a few (1), 610 cells as moderate (2), and > 10 as many (3). According to the staining intensity of Caveolin-1 at muscle tissue, a subjective evaluation was performed as; mild staining (1), moderate staining (2) and strong staining (3).
The mean value of ICC distribution was calculated 1.37 ± 0.54 in Group 1 and 2.13 ± 0.64 in Group 2 (p = 0.003), and the median value of ICC distribution was found 1 [1-3] in Group 1 and 2 [1-3] in Group 2 (p = 0.008). Median values for the intensity of staining with Caveolin-1 were found 2 [1-3] in the Group 1, and 2.5 [2-3] in the Group 2 (p = 0.025).
A decrease in ICC and Caveolin-1 levels support that there may be a relationship between ICC and Caveolin-1 for UPJO associated with signal transduction and peristalsis in urinary system.
比较两组分别患有和未患有肾盂输尿管连接部梗阻（UPJO）的儿科患者的输尿管肾盂连接部（UPJ）标本中，作为起搏细胞和信号分子的c-kit阳性间质 Cajal 样细胞（ICC）及小窝蛋白-1的蛋白水平。
我们回顾性评估了2005年至2012年间接受手术的45例儿科患者的UPJ标本。第1组包括37例因UPJO接受肾盂成形术的患者。8例因其他原因（肾肿瘤、创伤等）接受肾切除术且UPJ正常的患者被纳入第2组。标本用CD117和小窝蛋白-1抗体进行免疫组织化学检查。根据ICC的总数；0至5个细胞被视为少量（1），6至10个细胞为中等量（2），超过10个为大量（3）。根据小窝蛋白-1在肌肉组织中的染色强度，进行主观评估为；轻度染色（1），中度染色（2）和强染色（3）。
第1组ICC分布的平均值计算为1.37±0.54，第2组为2.13±0.64（p = 0.003），第1组ICC分布的中位数为1[1 - 3]，第2组为2[1 - 3]（p = 0.008）。第1组中小窝蛋白-1染色强度的中位数为2[1 - 3]，第2组为2.5[2 - 3]（p = 0.025）。
ICC和小窝蛋白-1水平的降低支持ICC和小窝蛋白-1之间可能存在与泌尿系统信号转导和蠕动相关的UPJO的关系。
