使用低能量 CID 谱鉴定双焦点糖型。

Identification of bifucosylated glycoforms using low-energy CID spectra.

机构信息

MS Proteomics Research Group, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudósok körútja 2, H-1117, Budapest, Hungary.

Károly Rácz School of PhD Studies, Semmelweis University, Üllői út 26, H-1085, Budapest, Hungary.

出版信息

J Mass Spectrom. 2019 Oct;54(10):817-822. doi: 10.1002/jms.4432. Epub 2019 Oct 9.

DOI:10.1002/jms.4432

PMID:31476246

Abstract

We have used tandem mass spectrometry (MS/MS)-based analysis of glycopeptides in order to identify the composition and structure of rare glycoforms. The results illustrate utility of low-energy MS/MS for structure identification. We have shown the presence of bifucosylated and trifucosylated glycoforms in human α-1-acid glycoprotein (AGP), a major plasma glycoprotein. Fucosylation in the case of AGP always occurs on the antennae; core fucosylation was not observed.

摘要

我们使用基于串联质谱（MS/MS）的糖肽分析方法来鉴定稀有糖型的组成和结构。结果表明低能 MS/MS 对于结构鉴定是有用的。我们已经在人α-1-酸性糖蛋白（AGP）中发现了双岩藻糖基化和三岩藻糖基化的糖型，AGP 是一种主要的血浆糖蛋白。AGP 中的岩藻糖基化总是发生在触角上；未观察到核心岩藻糖基化。