人血清和血浆中胎盘生长因子、可溶性 fms 样酪氨酸激酶 1 和可溶性 fms 样酪氨酸激酶 1e15a 的稳定性。
Stability of placental growth factor, soluble fms-like tyrosine kinase 1, and soluble fms-like tyrosine kinase 1 e15a in human serum and plasma.
机构信息
Department of Obstetrics & Gynaecology, Monash University, Clayton, Victoria, Australia.
Department of Obstetrics & Gynaecology, Monash University, Clayton, Victoria, Australia; Department of Obstetrics & Gynaecology, Monash Health, Clayton, Victoria, Australia.
出版信息
Placenta. 2019 Oct;86:1-3. doi: 10.1016/j.placenta.2019.08.001. Epub 2019 Aug 7.
Placental growth factor (PlGF), total soluble fms-like tyrosine-kinase 1 (sFlt-1) and its placental-specific variant, sFlt-1 e15a, show promise as biomarkers for the prediction and diagnosis of preeclampsia. This study describes the degradation of PlGF, sFlt-1 and sFlt-1 e15a within maternal serum and plasma to assist clinical implementation. Whole blood was refrigerated at 4 °C for up to 48 h prior to centrifugation for isolation of plasma and serum. PlGF and sFlt-1 were quantified using the B.R.A.H.M.S Kryptor Compact PLUS; sFlt-1 e15a via a custom ELISA. All three analytes are stable for at least 48 h at 4 °C. Serum and plasma performed comparably.
胎盘生长因子（PlGF）、总可溶性 fms 样酪氨酸激酶 1（sFlt-1）及其胎盘特异性变体 sFlt-1e15a，作为预测和诊断子痫前期的生物标志物具有一定的前景。本研究描述了母体血清和血浆中 PlGF、sFlt-1 和 sFlt-1e15a 的降解，以协助临床实施。全血在 4°C 下冷藏最多 48 小时，然后离心分离血浆和血清。使用 B.R.A.H.M.S Kryptor Compact PLUS 定量检测 PlGF 和 sFlt-1；通过定制 ELISA 检测 sFlt-1e15a。三种分析物在 4°C 下至少稳定 48 小时。血清和血浆的性能相当。
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