补充鱼油可减轻大气氧化剂总量对全身氧化应激的影响：一项随机、双盲、安慰剂对照试验。

Alleviated systemic oxidative stress effects of combined atmospheric oxidant capacity by fish oil supplementation: A randomized, double-blinded, placebo-controlled trial.

机构信息

School of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Laboratory of Health Technology Assessment, Fudan University, Shanghai, 200032, China.

School of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Laboratory of Health Technology Assessment, Fudan University, Shanghai, 200032, China; Shanghai Typhoon Institute/CMA, Shanghai Key Laboratory of Meteorology and Health, Shanghai, 200030, China.

出版信息

Ecotoxicol Environ Saf. 2019 Nov 30;184:109598. doi: 10.1016/j.ecoenv.2019.109598. Epub 2019 Aug 30.

DOI:10.1016/j.ecoenv.2019.109598

PMID:31476451

Abstract

BACKGROUND

Combined atmospheric oxidant capacity (O), represented by the sum of nitrogen dioxide (NO) and ozone (O), is an important hazardous property of outdoor air pollution mixture. It remains unknown whether its adverse effects can be ameliorated by dietary fish-oil supplementation.

OBJECTIVE

To assess the effects of fish-oil supplementation against oxidative stress induced by acute O exposure.

METHODS

We conducted a randomized, double-blinded and placebo-controlled study among 65 young adults in Shanghai, China between September 2017 and January 2018. We randomly assigned participants to receive either 2.5 g/day of fish oil or placebo, and conducted four repeated physical examinations during the last two months of treatments. O concentrations were calculated as the sum of hourly measurements of NO and O. We measured six biomarkers on systemic oxidative stress and antioxidant activity. Linear mixed-effect models were used to assess the short-term effects of O on biomarkers in each group.

RESULTS

During our study period, the 72-h average O concentration was 93.6 μg/m. Short-term exposure to O led to weaker changes in all biomarkers in the fish oil group than in the placebo group. Compared with the placebo group, for a 10-μg/m increase in O, there were smaller decrements in myeloperoxidase (MPO, difference = 5.92%, lag = 0-2 d, p = 0.03) and malondialdehyde (MDA, difference = 5.00%, lag = 1 d, p = 0.04) in the fish-oil group; there were also larger increments in total antioxidant capacity (TAC, difference = 16.33%, lag = 2 d, p = 0.02) and in glutathione peroxidase (GSH-Px, difference = 8.89%, lag = 0-2 d, p = 0.03) in the fish-oil group. The estimated differences for MPO were robust to adjustment for all co-pollutants and the differences for other biomarkers remained for some co-pollutants.

CONCLUSIONS

This trial provides first-hand evidence that dietary fish-oil supplementation may alleviate the systemic oxidative stress induced by O.

摘要

背景

大气氧化剂综合容量（O）由二氧化氮（NO）和臭氧（O）的总和表示，是室外空气污染混合物的一个重要有害特性。目前尚不清楚膳食鱼油补充是否可以减轻其不良影响。

目的

评估鱼油补充对急性 O 暴露引起的氧化应激的影响。

方法

我们于 2017 年 9 月至 2018 年 1 月在中国上海的 65 名年轻人中进行了一项随机、双盲、安慰剂对照研究。我们将参与者随机分配接受 2.5 g/天的鱼油或安慰剂，并在治疗的最后两个月进行了四次重复体检。O 浓度计算为每小时测量的 NO 和 O 的总和。我们测量了全身氧化应激和抗氧化活性的六个生物标志物。线性混合效应模型用于评估 O 对每组生物标志物的短期影响。

结果

在我们的研究期间，72 小时平均 O 浓度为 93.6μg/m。短期暴露于 O 导致鱼油组的所有生物标志物的变化均弱于安慰剂组。与安慰剂组相比，O 增加 10μg/m，鱼油组髓过氧化物酶（MPO，差异=5.92%，滞后=0-2d，p=0.03）和丙二醛（MDA，差异=5.00%，滞后=1d，p=0.04）的降幅较小；总抗氧化能力（TAC，差异=16.33%，滞后=2d，p=0.02）和谷胱甘肽过氧化物酶（GSH-Px，差异=8.89%，滞后=0-2d，p=0.03）的增幅较大。MPO 的估计差异在调整所有共污染物后仍然稳健，其他生物标志物的差异仍然存在于一些共污染物中。