Department of Neurology, Uonuma Institute of Community Medicine, Niigata University Medical and Dental Hospital, Japan.
Department of Pathology, Brain Research Institute, Niigata University, Japan.
Auton Neurosci. 2019 Nov;221:102583. doi: 10.1016/j.autneu.2019.102583. Epub 2019 Aug 22.
This study aimed to determine whether enteric neurons are involved in multiple system atrophy (MSA). Four-μm-thick slices of small intestine were prepared from 10%-formalin-fixed and paraffin-embedded materials obtained from autopsied cases. Enteric neurons were stained using an anti-peripherin antibody. Immunostaining of phosphorylated α-synuclein was also performed. Areas of the cytoplasm and nucleus that showed nucleoli were measured using computer software. Both areas of myenteric neurons were significantly smaller in MSA cases (n = 3) than in control subjects (n = 3) (P < 0.0001); however, no deposits of phosphorylated α-synuclein were observed. These findings suggest that myenteric neurons in MSA are affected independent of α-synuclein accumulation.
本研究旨在确定肠神经元是否参与多系统萎缩（MSA）。从小肠的 10％福尔马林固定和石蜡包埋材料中制备 4μm 厚的切片。使用抗周围蛋白抗体对肠神经元进行染色。还进行了磷酸化α-突触核蛋白的免疫染色。使用计算机软件测量显示核仁的细胞质和细胞核区域。与对照组（n=3）相比，MSA 病例（n=3）的肌间神经元的细胞质和细胞核区域明显更小（P<0.0001）；然而，未观察到磷酸化α-突触核蛋白的沉积。这些发现表明，MSA 中的肌间神经元受到影响与α-突触核蛋白的积累无关。
