Laboratory for Genome Engineering and Synthetic Biology, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia.
Plant Genetics Lab, TERRA Research and Teaching Centre, Gembloux Agro BioTech, University of Liège, Passage des Déportés 2, 5030 Gembloux, Belgium.
Trends Biotechnol. 2020 Mar;38(3):236-240. doi: 10.1016/j.tibtech.2019.08.001. Epub 2019 Aug 30.
Directed evolution involves generating diverse sequence variants of a gene of interest to produce a desirable trait under selective pressure. CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9) systems can be programmed to target any genomic locus and perform targeted directed evolution. Here, we discuss the opportunities and challenges of this emerging platform for targeted crop improvement.
定向进化涉及在选择压力下生成感兴趣基因的多种序列变体，以产生所需性状。CRISPR-Cas9（成簇规律间隔短回文重复序列-CRISPR 相关蛋白 9）系统可以被编程靶向任何基因组位点并进行靶向定向进化。在这里，我们讨论了这个新兴的靶向作物改良平台的机会和挑战。
