Saydjari R, Townsend C M, Barranco S C, Thompson J C
Department of Surgery, University of Texas Medical Branch, Galveston.
Invest New Drugs. 1988 Dec;6(4):265-72. doi: 10.1007/BF00173644.
We have previously reported that the in vitro growth of MC-26 mouse colon cancer and H2T hamster pancreatic cancer cells are inhibited by cyclosporine (CsA) and alpha-difluoromethylornithine (DFMO). The present study was designed to investigate the effects of these two drugs on the two experimental tumors (MC-26 and H2T) growing in vivo. Forty-eight male Balb/c mice or Syrian golden hamsters were inoculated with MC-26 (250,000) or H2T (500,000) cells, respectively, and then were randomized into four groups of 12 each: group I was control; group II received CsA; group III received DFMO; group IV received a combination of CsA and DFMO. MC-26 tumors were significantly more sensitive than H2T tumors to the effects of CsA and DFMO. MC-26 tumor growth and tumor weight, as well as the tumor content of DNA, RNA, and protein were all significantly more reduced by CsA and DFMO than were the H2T tumors. Our present study shows that both CsA and DFMO are potent inhibitors of MC-26 colon carcinoma growth in vivo, though DFMO is more than twice as effective as CsA. DFMO also produced greater reductions in the tumor content of DNA, RNA, and protein than did CsA. DFMO significantly decreased the concentrations of polyamines in both H2T and MC-26 tumors; the MC-26 tumors were affected to a greater degree.
我们之前曾报道，环孢素（CsA）和α-二氟甲基鸟氨酸（DFMO）可抑制MC-26小鼠结肠癌和H2T仓鼠胰腺癌细胞的体外生长。本研究旨在调查这两种药物对两种体内生长的实验性肿瘤（MC-26和H2T）的影响。48只雄性Balb/c小鼠或叙利亚金仓鼠分别接种MC-26（250,000个）或H2T（500,000个）细胞，然后随机分为四组，每组12只：第一组为对照组；第二组接受CsA；第三组接受DFMO；第四组接受CsA和DFMO联合治疗。MC-26肿瘤对CsA和DFMO的作用比H2T肿瘤更敏感。与H2T肿瘤相比，CsA和DFMO对MC-26肿瘤生长、肿瘤重量以及肿瘤中DNA、RNA和蛋白质含量的降低幅度均更大。我们目前的研究表明，CsA和DFMO都是体内MC-26结肠癌生长的有效抑制剂，尽管DFMO的效果是CsA的两倍多。DFMO对肿瘤中DNA、RNA和蛋白质含量的降低幅度也比CsA更大。DFMO显著降低了H2T和MC-26肿瘤中多胺的浓度；MC-26肿瘤受到的影响更大。
