Townsend C M, Franklin R B, Gelder F B, Glass E, Thompson J C
Surgery. 1982 Jul;92(1):72-8.
We report here the first development of a continuous cell line in tissue culture of an animal pancreatic duct adenocarcinoma that is histologically similar to human pancreatic duct adenocarcinoma. A primary pancreatic duct adenocarcinoma, induced in a male Syrian golden hamster after 23 weeks of weekly subcutaneous injection of N-nitrobis (2-hydroxypropyl)amine, was minced and injected subcutaneously into three hamsters. After 8 weeks, a single tumor was apparent. Subsequent passages of fragments into the cheek pouches were performed at 3- to 4-week intervals. After five passages, minced fragments of a tumor were placed in tissue culture. Colonies appeared by 7 days; an epitheloid cell line, without fibroblasts, was established by 60 days. Single-cell suspensions, injected into hamster cheek pouches or subcutaneously, produced tumors in a dose-dependent fashion. Spent culture medium of tissue culture cells and saline extracts of freshly excised tumors contained pancreatic oncofetal antigen-like activity.
我们在此报告首次在动物胰腺导管腺癌的组织培养中培养出连续细胞系，该细胞系在组织学上与人类胰腺导管腺癌相似。一只雄性叙利亚金仓鼠在每周皮下注射N-亚硝基双（2-羟丙基）胺23周后诱发原发性胰腺导管腺癌，将其切碎并皮下注射到三只仓鼠体内。8周后，出现了一个单一肿瘤。随后每隔3至4周将肿瘤碎片接种到颊囊中。经过五次传代后，将肿瘤切碎的碎片进行组织培养。7天时出现菌落；60天时建立了无成纤维细胞的上皮样细胞系。单细胞悬液注射到仓鼠颊囊或皮下，以剂量依赖方式产生肿瘤。组织培养细胞的用过的培养基和新鲜切除肿瘤的盐水提取物含有胰腺癌胚抗原样活性。
