Matos Andressa Maria Rorato Nascimento de, Lavorente Fernanda Louise Pereira, Lorenzetti Elis, Meira Filho Mário Roberto Castro, Nóbrega Daniela Farias da, Chryssafidis Andreas Lazaros, Oliveira Admilton Gonçalves de, Domit Camila, Bracarense Ana Paula Frederico Rodrigues Loureiro
Laboratório de Patologia Animal, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
Programa de Pós-graduação em Ciência Animal, Centro de Ciências Agrárias, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
Rev Bras Parasitol Vet. 2019 Aug 29;28(3):367-375. doi: 10.1590/S1984-29612019025.
Renicolids are parasites that inhabit the renal tubules and ureters of molluscivorous and piscivorous birds. Puffinus puffinus is a migratory seabird that was identified as the definitive host of Renicola spp. Studies focusing on the renicolid species and the resulting renal lesions are valuable for their association with causes of stranding in seabirds. The aim of this study was to identify the renicolid trematodes and evaluate the histological findings in two P. puffinus stranded on the coast of Paraná state, Brazil. The parasites were evaluated by histologic, ultrastructural and molecular assays, while tissue changes were analyzed by histologic methods. The morphological and morphometrical characteristics of the parasites, along with polymerase chain reaction and sequencing assays (ribosomal and mitochondrial regions), identified the species as Renicola sloanei. The results also suggest that this helminth can be the adult form of Cercaria pythionike. The dilation of collecting ducts was the main histological finding in the kidneys. In conclusion, R. sloanei was identified, and for the first time, P. puffinus was described as a host of this digenean inducing mild renal changes.
肾居吸虫是寄生于食软体动物和食鱼鸟类肾小管和输尿管的寄生虫。普通海鸦是一种迁徙海鸟，被确定为肾居吸虫属的终末宿主。针对肾居吸虫种类及其所致肾脏病变的研究，因其与海鸟搁浅原因的关联而具有重要价值。本研究的目的是鉴定肾居吸虫，并评估在巴西巴拉那州海岸搁浅的两只普通海鸦的组织学发现。通过组织学、超微结构和分子检测对寄生虫进行评估，同时采用组织学方法分析组织变化。寄生虫的形态和形态测量特征，以及聚合酶链反应和测序检测（核糖体和线粒体区域），将该物种鉴定为斯氏肾居吸虫。结果还表明，这种蠕虫可能是毕氏尾蚴的成虫形态。集合管扩张是肾脏的主要组织学发现。总之，鉴定出了斯氏肾居吸虫，并且首次描述普通海鸦是这种复殖吸虫的宿主，该吸虫可引起轻度肾脏变化。
