Macedo Iara Tersia Freitas, Oliveira Lorena Mayana Beserra de, André Weibson Paz Pinheiro, Araújo Filho José Vilemar de, Santos Jéssica Maria Leite Dos, Rondon Fernanda Cristina Macedo, Ribeiro Wesley Lyeverton Correia, Camurça-Vasconcelos Ana Lourdes Fernandes, Oliveira Erick Falcão de, Paula Haroldo César Beserra de, Bevilaqua Claudia Maria Leal
Programa de Pós-graduação em Ciências Veterinárias, Laboratório de Doenças Parasitárias, Faculdade de Veterinária, Universidade Estadual do Ceará - UECE, Fortaleza, CE, Brasil.
Centro Universitário - INTA-Uninta, Sobral, CE, Brasil.
Rev Bras Parasitol Vet. 2019 Aug 29;28(3):522-527. doi: 10.1590/S1984-29612019065.
The anthelmintic resistance stimulated the search for strategies for controlling gastrointestinal nematodes, including the use of free essential oils or its nanoemulsion. This study evaluated the anthelmintic efficacy of Cymbopogon citratus essential oil (CcEO) and C. citratus essential oil nanoemulsion (CcEOn). Pysicochemical analyses were performed. The in vitro effect was determined using the egg hatch test (EHT) on Haemonchus contortus and in vivo effect was evaluated in sheep infected with gastrointestinal nematodes. The animals were treated with CcEO (500 mg/kg) or CcEOn (450 mg/kg) for the fecal egg count (FEC) and the determination of worm burden. The main component of CcEO was citral. The CcEO content in the nanoemulsion was 20% (v/v), and the mean particle size was 248 nm. In EHT, CcEO and CcEOn (1.25 mg/mL) inhibited larval hatching by 98.4 and 97.1%, respectively. Three animals treated with CcEO died whereas in the group treated with CcEOn one animal died. The FEC and total worm burden of the treated groups did not differ from the negative control (p>0.05). The CcEOn showed efficacy only on H. contortus (p<0.05). In conclusion, nanoencapsulation reduced toxicity and increased efficacy on H. contortus.
抗蠕虫药耐药性促使人们寻找控制胃肠道线虫的策略，包括使用游离精油或其纳米乳剂。本研究评估了柠檬香茅精油（CcEO）和柠檬香茅精油纳米乳剂（CcEOn）的驱虫效果。进行了理化分析。使用捻转血矛线虫的虫卵孵化试验（EHT）测定体外效果，并在感染胃肠道线虫的绵羊中评估体内效果。用CcEO（500 mg/kg）或CcEOn（450 mg/kg）处理动物，以进行粪便虫卵计数（FEC）和确定虫负荷。CcEO的主要成分是柠檬醛。纳米乳剂中CcEO的含量为20%（v/v），平均粒径为248 nm。在EHT中，CcEO和CcEOn（1.25 mg/mL）分别抑制幼虫孵化98.4%和97.1%。三只接受CcEO治疗的动物死亡，而在接受CcEOn治疗的组中有一只动物死亡。治疗组的FEC和总虫负荷与阴性对照无差异（p>0.05）。CcEOn仅对捻转血矛线虫显示出疗效（p<0.05）。总之，纳米包封降低了毒性并提高了对捻转血矛线虫的疗效。
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