Campos Viviane Souza, Rocha Letícia Oliveira da, Leandro Hassan Jerdy, Pontes Teresa, Oliveira Fábio Conceição de, Carvalho Eulógio Carlos Queiroz de, Glória Leonardo Siqueira, Santos Clóvis de Paula
Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF, Campos dos Goytacazes, RJ, Brasil.
Laboratório de Patologia Veterinária, Centro de Ciências Tecnológica e Agropecuárias, Universidade Estadual do Norte Fluminense - UENF, Campos dos Goytacazes, RJ, Brasil.
Rev Bras Parasitol Vet. 2024 Dec 20;33(4):e014224. doi: 10.1590/S1984-29612024079. eCollection 2024.
This paper describes a novel in vivo study of Cymbopogon citratus (lemon grass) to assess its anthelmintic activity. To this end, C57BL/6 mice were separated into three groups: G1: uninfected; G2: negative control infected with Heligmosomoides polygyrus bakeri and administered with 3% dimethyl sulfoxide (DMSO); and G3: infected with H. polygyrus bakeri and treated with C. citratus aqueous extract (50mg/kg). The extract and H. polygyrus bakeri were administered via gavage and the anatomo-histopathological evaluation of the animals took place after necropsy and organ removal. In addition, the number of eggs per gram of feces (epg) and of adult parasites in the small intestine of each animal, as well as blood cell counts, were assessed. The in vivo assay revealed a reduction in the epg (54%), number of adult nematodes (89%), number of eosinophils, and intestinal lesions in mice treated with C. citratus. These results suggest that the crude aqueous extract of C. citratus at the dose evaluated here has anthelmintic and possibly anti-inflammatory properties, given its effectiveness against gastrointestinal H. polygyrus bakeri nematodes and the recovery of damaged tissues. Therefore, this plant shows potential to control gastrointestinal nematodes.
本文描述了一项关于柠檬草的新型体内研究，以评估其驱虫活性。为此，将C57BL/6小鼠分为三组：G1：未感染组；G2：感染巴氏多毛圆线虫的阴性对照组，给予3%二甲基亚砜（DMSO）；G3：感染巴氏多毛圆线虫并用柠檬草水提取物（50mg/kg）治疗的组。提取物和巴氏多毛圆线虫通过灌胃给药，在尸检和摘除器官后对动物进行解剖组织病理学评估。此外，还评估了每克粪便中的虫卵数（epg）、每只动物小肠内成虫寄生虫的数量以及血细胞计数。体内试验显示，用柠檬草治疗的小鼠的epg（54%）、成虫线虫数量（89%）、嗜酸性粒细胞数量和肠道病变均有所减少。这些结果表明，在此评估剂量下，柠檬草粗水提取物具有驱虫作用，并且可能具有抗炎特性，因为它对胃肠道巴氏多毛圆线虫有效且能使受损组织恢复。因此，这种植物显示出控制胃肠道线虫的潜力。
