Sena Luana Samara Balduíno de, Santos Hellen Bandeira de Pontes, Amaral Marina Gonçalves do, Gordón-Núñez Manuel Antonio, Alves Pollianna Muniz, Nonaka Cassiano Francisco Weege
Universidade Estadual da Paraíba - UEPB, Department of Dentistry, Campina Grande, PB, Brazil.
Universidade Federal do Rio Grande do Norte - UFRN, Department of Oral Pathology, Natal, RN, Brazil.
Braz Oral Res. 2019 Aug 29;33:e085. doi: 10.1590/1807-3107bor-2019.vol33.0085.
The aim of this study was to evaluate the immunoexpression of human leukocyte antigen-DR (HLA-DR) in actinic cheilitis (AC) and lower lip squamous cell carcinoma (LLSCC), and to correlate the findings with clinical (tumor size/extent, regional lymph node metastasis, and clinical stage) and histopathological (grade of epithelial dysplasia and inflammatory infiltrate for AC and histopathological grade of malignancy for LLSCC) parameters. Twenty-four AC and 48 LLSCC cases (24 with regional nodal metastasis and 24 without regional nodal metastasis) were selected. The scores of immunopositive cells for HLA-DR in the epithelial component of the lesions were assessed and the results were analyzed statistically using the nonparametric Mann-Whitney test. Epithelial expression of HLA-DR was observed in only five (20.8%) cases of AC (two low-grade and three high-grade lesions), with a very low median score of immunopositivity. By contrast, expression of HLA-DR was found in most LLSCC (97.9%), with a relatively high median score of positive cells. The score of HLA-DR-positive cells tended to be higher in tumors with regional lymph node metastasis, tumors in advanced clinical stages, and low-grade tumors, but the difference was not statistically significant (p > 0.05). In addition, there was a tendency towards higher expression of HLA-DR in highly/moderately keratinized tumors, and tumors with little/moderate nuclear pleomorphism (p > 0.05). The results suggest a potential role of HLA-DR in lip carcinogenesis, particularly in the development and progression of LLSCC. The expression of this protein can be related to the degree of cell differentiation in these tumors.
本研究的目的是评估人类白细胞抗原-DR（HLA-DR）在光化性唇炎（AC）和下唇鳞状细胞癌（LLSCC）中的免疫表达，并将研究结果与临床（肿瘤大小/范围、区域淋巴结转移和临床分期）以及组织病理学（AC的上皮发育异常等级和炎症浸润，LLSCC的组织病理学恶性等级）参数相关联。选取了24例AC和48例LLSCC病例（24例有区域淋巴结转移，24例无区域淋巴结转移）。评估病变上皮成分中HLA-DR免疫阳性细胞的评分，并使用非参数曼-惠特尼检验对结果进行统计学分析。仅在5例（20.8%）AC病例（2例低级别和3例高级别病变）中观察到HLA-DR的上皮表达，免疫阳性中位数评分非常低。相比之下，大多数LLSCC（97.9%）中发现了HLA-DR的表达，阳性细胞的中位数评分相对较高。HLA-DR阳性细胞的评分在有区域淋巴结转移的肿瘤、临床晚期肿瘤和低级别肿瘤中往往较高，但差异无统计学意义（p>0.05）。此外，在高度/中度角化肿瘤以及核多形性小/中度的肿瘤中，HLA-DR有表达升高的趋势（p>0.05）。结果表明HLA-DR在唇部致癌过程中具有潜在作用，尤其是在LLSCC的发生和发展中。该蛋白的表达可能与这些肿瘤中的细胞分化程度有关。
