Department of Epidemiology and Biostatistics, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China.
State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
J Cell Mol Med. 2019 Nov;23(11):7261-7267. doi: 10.1111/jcmm.14532. Epub 2019 Sep 4.
Many studies have shown that there were similarity between tumorigenesis and gametogenesis. Our previous work found that cancer-testis (CT) genes could serve as a novel source of candidate of cancer. Here, by analysing The Cancer Genome Atlas (TCGA) database, we characterized a CT gene, SPANXC, which is expressed only in testis. The SPANXC was reactivated in lung adenocarcinoma (LUAD) tissues. And the expression of SPANXC was associated with prognosis of LUAD. We also found that the activation of SPANXC was due to the promoter hypomethylation of SPANXC. Moreover, SPANXC could modulate cell metastasis both in vitro and in vivo. Mechanistically, we found that SPANXC could bind to ROCK1, a metastasis-related gene, and thus SPANXC may regulate cell metastasis partly through interaction with ROCK1 in LUAD. Together, our results demonstrated that the CT expression pattern of SPANXC served as a crucial role in metastasis of LUAD. And these data further corroborated the resemblance between processes of germ cell development and tumorigenesis, including migration and invasion.
许多研究表明肿瘤发生和配子发生之间存在相似性。我们之前的工作发现，癌症睾丸（CT）基因可以作为癌症候选物的新来源。在这里，通过分析癌症基因组图谱（TCGA）数据库，我们鉴定了一个仅在睾丸中表达的 CT 基因 SPANXC。SPANXC 在肺腺癌（LUAD）组织中被重新激活。并且 SPANXC 的表达与 LUAD 的预后相关。我们还发现 SPANXC 的激活是由于 SPANXC 启动子的低甲基化。此外，SPANXC 可以在体外和体内调节细胞转移。在机制上，我们发现 SPANXC 可以与 ROCK1 结合，ROCK1 是一个与转移相关的基因，因此 SPANXC 可能通过与 LUAD 中的 ROCK1 相互作用部分调节细胞转移。总之，我们的结果表明，SPANXC 的 CT 表达模式在 LUAD 的转移中起着关键作用。这些数据进一步证实了生殖细胞发育和肿瘤发生过程之间的相似性，包括迁移和侵袭。
