Bai Bijie, Wang Hongda, Liu Tairan, Rivenson Yair, FitzGerald John, Ozcan Aydogan
Electrical and Computer Engineering Department, University of California, Los Angeles, Los Angeles, California.
Bioengineering Department, University of California, Los Angeles, Los Angeles, California.
J Biophotonics. 2020 Jan;13(1):e201960036. doi: 10.1002/jbio.201960036. Epub 2019 Oct 9.
Pathological crystal identification is routinely practiced in rheumatology for diagnosing arthritis disease such as gout, and relies on polarized light microscopy as the gold standard method used by medical professionals. Here, we present a single-shot computational polarized light microscopy method that reconstructs the transmittance, retardance and slow-axis orientation of a birefringent sample using a single image captured with a pixelated-polarizer camera. This method is fast, simple-to-operate and compatible with all the existing standard microscopes without extensive or costly modifications. We demonstrated the success of our method by imaging three different types of crystals found in synovial fluid and reconstructed the birefringence information of these samples using a single image, without being affected by the orientation of individual crystals within the sample field-of-view. We believe this technique will provide improved sensitivity, specificity and speed, all at low cost, for clinical diagnosis of crystals found in synovial fluid and other bodily fluids.
在风湿病学中，病理晶体鉴定是诊断痛风等关节炎疾病的常规操作，并且依赖偏光显微镜作为医学专业人员使用的金标准方法。在此，我们提出一种单次计算偏光显微镜方法，该方法使用像素化偏振器相机拍摄的单张图像重建双折射样品的透过率、相位延迟和慢轴方向。此方法快速、易于操作，并且与所有现有的标准显微镜兼容，无需进行大量或昂贵的修改。我们通过对滑液中发现的三种不同类型的晶体进行成像，证明了我们方法的成功，并使用单张图像重建了这些样品的双折射信息，而不受样品视场内单个晶体方向的影响。我们相信，这项技术将以低成本为滑液和其他体液中发现的晶体的临床诊断提供更高的灵敏度、特异性和速度。
