Buneri Islam Dad, Yousuf Masarrat, Attaullah Mohammad, Afridi Saifullah, Anjum Syed Ishtiaq, Rana Habibullah, Ahmad Naveed, Amin Muhammad, Tahir Muhammad, Ansari Mohammad Javed
Toxicology Laboratory, Department of Zoology, University of Karachi, Karachi, Pakistan.
Department of Zoology, Shaheed BB University, Sheringal, Upper Dir, Pakistan.
Saudi J Biol Sci. 2019 Feb;26(2):281-285. doi: 10.1016/j.sjbs.2017.06.005. Epub 2017 Jun 10.
(deodar) is practically used, as insect repellent, in the northern areas of Pakistan but no data available therefore this study was conducted to evaluate the effectiveness of deodar oil as an alternate of conventional insecticides against the larval pest stage of mealworm beetle (), by feeding method. The aim of the study was to investigate the effectiveness of deodar oil as an alternate of conventional insecticides against the larval pest stage of mealworm beetle (), by feeding method. All tested chemicals showed efficacy against the pests. The LC was determined by probit analysis and was found to be 3.41, 0.086 and 0.023% of larvae treated with deodar oil, Carbosulfan and Imidacloprid respectively The LC treated larvae were subjected to the evaluation of protein activity, qualitatively and quantitatively. The protein level in tested insects was enhanced when treated with Imidacloprid, Carbosulfan and deodar oil. The electrophoretic profile of treated insects showed more bands in insects treated with oil. This electrophoretic profile appeared in 4, 5, 7 and 8 bands for tested chemicals including control. Antifeedant activity was observed for as larvae were deterred to feed on the food found in the container.
在巴基斯坦北部地区,(雪松)实际上被用作驱虫剂,但尚无相关数据。因此,本研究通过喂食法评估雪松油作为传统杀虫剂替代品对黄粉虫幼虫害虫阶段的有效性。本研究的目的是通过喂食法研究雪松油作为传统杀虫剂替代品对黄粉虫幼虫害虫阶段的有效性。所有测试化学品均显示出对害虫的有效性。通过概率分析确定LC,发现用雪松油、丁硫克百威和吡虫啉处理的幼虫的LC分别为3.41%、0.086%和0.023%。对经LC处理的幼虫进行蛋白质活性的定性和定量评估。用吡虫啉、丁硫克百威和雪松油处理后,受试昆虫体内的蛋白质水平有所提高。经处理昆虫的电泳图谱显示,用雪松油处理的昆虫中有更多条带。包括对照在内的受试化学品的电泳图谱在4、5、7和8条带中出现。观察到雪松油具有拒食活性,因为幼虫被阻止取食容器中的食物。