通过用含有弗氏白血病病毒env基因的质粒转染使肿瘤细胞异种化。
Xenogenization of tumor cells by transfection with plasmid containing env gene of Friend leukemia virus.
作者信息
Sugiura C, Itaya T, Kondoh N, Oikawa T, Kuzumaki N, Takeichi N, Hosokawa M, Kobayashi H
机构信息
Laboratory of Pathology, Hokkaido University School of Medicine, Sapporo.
出版信息
Jpn J Cancer Res. 1988 Dec;79(12):1259-63. doi: 10.1111/j.1349-7006.1988.tb01553.x.
A rat hepatocellular carcinoma cell line (cKDH-8 cl-11) showed decreased tumorigenicity after transfection with an envelope gene derived from a Friend leukemia virus (FV-env gene). FV-env gene product was found by indirect immunofluorescence staining to be expressed on the cell surface of the FV-env gene-transfected cells. The FV-env-transfected cells (FV-env cKDH-8), however, grew well in X-irradiated immunosuppressed rats, indicating that the reduction in tumorigenicity of the transfected cells is based on immunological reaction in the host. The rats which rejected FV-env cKDH-8 cells showed resistance to rechallenge with the parent cKDH-8 cl-11 tumor cells. These results suggest that the FV-env gene product may elicit antitumor immunity against FV-env cKDH-8 cells in a host with a resultant reduction in the tumorigenicity of these cells.
一种大鼠肝癌细胞系（cKDH-8 cl-11）在用源自弗瑞德白血病病毒的包膜基因（FV-env基因）转染后，致瘤性降低。通过间接免疫荧光染色发现，FV-env基因产物在FV-env基因转染细胞的细胞表面表达。然而，FV-env转染细胞（FV-env cKDH-8）在经X射线照射的免疫抑制大鼠中生长良好，这表明转染细胞致瘤性的降低是基于宿主中的免疫反应。排斥FV-env cKDH-8细胞的大鼠对亲本cKDH-8 cl-11肿瘤细胞的再次攻击表现出抗性。这些结果表明，FV-env基因产物可能在宿主体内引发针对FV-env cKDH-8细胞的抗肿瘤免疫，从而导致这些细胞的致瘤性降低。
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