Sugiura C, Itaya T, Kondoh N, Oikawa T, Kuzumaki N, Takeichi N, Hosokawa M, Kobayashi H
Laboratory of Pathology, Hokkaido University School of Medicine, Sapporo.
Jpn J Cancer Res. 1988 Dec;79(12):1259-63. doi: 10.1111/j.1349-7006.1988.tb01553.x.
A rat hepatocellular carcinoma cell line (cKDH-8 cl-11) showed decreased tumorigenicity after transfection with an envelope gene derived from a Friend leukemia virus (FV-env gene). FV-env gene product was found by indirect immunofluorescence staining to be expressed on the cell surface of the FV-env gene-transfected cells. The FV-env-transfected cells (FV-env cKDH-8), however, grew well in X-irradiated immunosuppressed rats, indicating that the reduction in tumorigenicity of the transfected cells is based on immunological reaction in the host. The rats which rejected FV-env cKDH-8 cells showed resistance to rechallenge with the parent cKDH-8 cl-11 tumor cells. These results suggest that the FV-env gene product may elicit antitumor immunity against FV-env cKDH-8 cells in a host with a resultant reduction in the tumorigenicity of these cells.
一种大鼠肝癌细胞系（cKDH-8 cl-11）在用源自弗瑞德白血病病毒的包膜基因（FV-env基因）转染后，致瘤性降低。通过间接免疫荧光染色发现，FV-env基因产物在FV-env基因转染细胞的细胞表面表达。然而，FV-env转染细胞（FV-env cKDH-8）在经X射线照射的免疫抑制大鼠中生长良好，这表明转染细胞致瘤性的降低是基于宿主中的免疫反应。排斥FV-env cKDH-8细胞的大鼠对亲本cKDH-8 cl-11肿瘤细胞的再次攻击表现出抗性。这些结果表明，FV-env基因产物可能在宿主体内引发针对FV-env cKDH-8细胞的抗肿瘤免疫，从而导致这些细胞的致瘤性降低。
