miR-9 通过 TGF-β1/Smads 信号通路对大鼠心肌纤维化的影响。
Effect of miR-9 on myocardial fibrosis in rats via TGF-β1/Smads signaling pathway.
机构信息
Department of Cardiology, Yantai Hospital of Traditional Chinese Medicine, Yantai, China.
出版信息
Eur Rev Med Pharmacol Sci. 2019 Aug;23(16):7083-7088. doi: 10.26355/eurrev_201908_18752.
OBJECTIVE
To observe the effect of micro ribonucleic acid (miR)-9 on the myocardial fibrosis (MF) in rats and explore its mechanism of action.
MATERIALS AND METHODS
A total of 30 rats were selected to prepare the models of MF and randomly divided into Sham group, Model group and MiR-9 group. The rat heart weight (HW)/body weight (BW) and left ventricular weight (LVW)/BW were determined. The content of hydroxyproline (Hyp) in the myocardial tissues of left ventricle was measured using a spectrophotometer. Enzyme-linked immunosorbent assay (ELISA) was performed to determine the levels of type I and type III collagens in myocardial tissues, and the level of miR-9 in myocardial tissues was determined via reverse transcription-polymerase chain reaction (RT-PCR). Finally, Western blotting was conducted to measure the levels of transforming growth factor-β1 (TGF-β1) and Smads2 proteins.
RESULTS
Compared with Sham group, Model group exhibited increased HW/BW and LVW/BW and markedly raised Hyp content and type I collagen, type III collagen, TGF-β1 protein and Smads2 protein levels, but significantly declined miR-9 expression levels in rats. In MiR-9 group, the rats had substantially decreased HW/BW, LVW/BW, Hyp content, and type I and type III collagen levels as well as lowered TGF-β1 protein and Smads2 protein levels.
CONCLUSIONS
MiR-9 can alleviate the MF in rats by inhibiting the TGF-β1/Smads signaling pathway.
目的
观察微小 RNA-9（miR-9）对大鼠心肌纤维化（MF）的影响，并探讨其作用机制。
材料与方法
选择 30 只大鼠制备 MF 模型，并随机分为假手术组、模型组和 miR-9 组。测定大鼠心脏重量（HW）/体质量（BW）和左心室重量（LVW）/BW。分光光度计测定左心室心肌组织羟脯氨酸（Hyp）含量。酶联免疫吸附试验（ELISA）测定心肌组织 I 型和 III 型胶原含量，逆转录-聚合酶链反应（RT-PCR）测定心肌组织 miR-9 水平。最后，通过 Western blot 测定转化生长因子-β1（TGF-β1）和 Smads2 蛋白水平。
结果
与假手术组相比，模型组大鼠 HW/BW 和 LVW/BW 增加，Hyp 含量以及 I 型和 III 型胶原、TGF-β1 蛋白和 Smads2 蛋白水平升高，而 miR-9 表达水平降低。在 miR-9 组，大鼠 HW/BW、LVW/BW、Hyp 含量、I 型和 III 型胶原水平以及 TGF-β1 蛋白和 Smads2 蛋白水平均显著降低。
结论
miR-9 通过抑制 TGF-β1/Smads 信号通路减轻大鼠 MF。
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