SCN5A 基因突变与心房颤动发病的相关性。

Correlations of SCN5A gene polymorphisms with onset of atrial fibrillation.

机构信息

Department of Emergency, Tianjin Union Medical Center, Tianjin, China.

出版信息

Eur Rev Med Pharmacol Sci. 2019 Aug;23(16):7089-7097. doi: 10.26355/eurrev_201908_18754.

DOI:10.26355/eurrev_201908_18754

PMID:31486511

Abstract

OBJECTIVE

The aim of this study was to analyze the correlations of the sodium channel, voltage-gated, type V, alpha subunit (SCN5A) gene polymorphisms with the onset of atrial fibrillation (AF), and its clinical significance.

PATIENTS AND METHODS

The quantitative Real Time-Polymerase Chain Reaction (QRT-PCR) amplification and the TaqMan analysis were utilized to analyze the composition of SCN5A genotypes and alleles in the peripheral blood mononuclear cells of 48 normal controls and 115 AF patients. Meanwhile, the differences in single nucleotide polymorphisms (SNPs), 1673 A>G, and 3666+69 G>C, between the AF group and the control group were analyzed using the χ2-test. The high-risk factors influencing the AF attack were analyzed via logistic regression analysis, as well as univariate and multivariate analyses. In addition, the correlations of gene polymorphisms with high-risk factors (drinking and hypertension) for AF were verified by the χ2-test.

RESULTS

There were statistically significant differences in the incidence rate of hypertension, the times of smoking and drinking, and the frequencies of palpitation and syncope between the AF group and the control group (p<0.05). The composition of genotypes and alleles of 1673 A>G and 3666+69 G>C in the AF group was significantly different from that of the control group (p<0.05). According to the results of the logistic regression analysis, as well as the univariate and multivariate analyses, drinking, and hypertension were associated with the occurrence of AF (p<0.05). Furthermore, statistically significant differences were observed in the composition of the gene polymorphisms 1673 A>G and 3666+69 G>C between patients with and without histories of drinking and hypertension (p<0.05).

CONCLUSIONS

There are significant differences in SCN5A gene polymorphisms 1673 A>G and 3666+69 G>C between AF patients and normal controls. Moreover, drinking and hypertension can influence the changes in the gene polymorphisms.

摘要

目的

本研究旨在分析电压门控钠离子通道 V 型 α 亚基（SCN5A）基因多态性与心房颤动（AF）发病的相关性及其临床意义。

方法

利用定量实时聚合酶链反应（QRT-PCR）扩增和 TaqMan 分析方法，检测外周血单个核细胞中 SCN5A 基因型和等位基因在 48 例正常对照者和 115 例 AF 患者中的构成。同时，采用 χ2 检验分析 AF 组与对照组之间单核苷酸多态性（SNP）1673 A>G 和 3666+69 G>C 的差异。采用 logistic 回归分析、单因素和多因素分析，分析影响 AF 发作的高危因素。另外，通过 χ2 检验验证基因多态性与 AF 的高危因素（饮酒和高血压）的相关性。

结果

AF 组和对照组的高血压发生率、吸烟和饮酒次数、心悸和晕厥发作频率差异均有统计学意义（p<0.05）。AF 组 1673 A>G 和 3666+69 G>C 的基因型和等位基因构成与对照组差异有统计学意义（p<0.05）。根据 logistic 回归分析以及单因素和多因素分析结果，饮酒和高血压与 AF 的发生有关（p<0.05）。此外，在有饮酒和高血压病史的患者中，基因多态性 1673 A>G 和 3666+69 G>C 的构成差异有统计学意义（p<0.05）。