Buxser S, Vroegop S
Upjohn Company, Kalamazoo, Michigan 49001.
J Immunogenet. 1988 Feb-Jun;15(1-3):153-9. doi: 10.1111/j.1744-313x.1988.tb00417.x.
Staphylococcal enterotoxin B (SEB) is a T cell mitogen with properties different from the plant lectin mitogens. We examined the stimulation of mitogenesis induced by SEB in BALB/c mouse spleen cells and its relationship to major histocompatibility complex (MHC) and related cell surface proteins. Based on the ability of specific monoclonal antibodies to block mitogenesis, SEB stimulation appears to be more dependent on interaction with I-E than with I-A class II MHC molecules. Additionally, anti-L3T4, and possibly other antibodies specific for proteins related to the T cell receptor complex, were inhibitory. When A20 cells were treated with SEB and used to stimulate BALB/c spleen cells which were not otherwise exposed to SEB, the treated A20 cells were capable of stimulating mitogenesis of the BALB/c spleen cells. The data support the hypothesis that SEB stimulation is mediated primarily by interactions with class II MHC proteins and possibly proteins in the T cell receptor complex. We also observed that the presence of SEB in DBA/2 (Mlsa)-stimulated BALB/c (Mlsb) spleen cell cultures enhanced the BALB/c mitogenesis three-fold over the sum of the SEB-plus Mls-stimulated mitogenesis. These results suggest that SEB may be a useful tool for further exploration of the Mls response.
葡萄球菌肠毒素B（SEB）是一种T细胞有丝分裂原，其特性不同于植物凝集素类有丝分裂原。我们检测了SEB在BALB/c小鼠脾细胞中诱导的有丝分裂原刺激作用及其与主要组织相容性复合体（MHC）和相关细胞表面蛋白的关系。基于特异性单克隆抗体阻断有丝分裂原的能力，SEB刺激似乎更依赖于与I-E的相互作用，而不是与II类MHC分子I-A的相互作用。此外，抗L3T4以及可能其他针对与T细胞受体复合物相关蛋白的抗体具有抑制作用。当用SEB处理A20细胞并用于刺激未接触过SEB的BALB/c脾细胞时，经处理的A20细胞能够刺激BALB/c脾细胞的有丝分裂。这些数据支持了以下假设：SEB刺激主要是通过与II类MHC蛋白以及可能与T细胞受体复合物中的蛋白相互作用介导的。我们还观察到，在DBA/2（Mlsa）刺激的BALB/c（Mlsb）脾细胞培养物中SEB的存在，使BALB/c的有丝分裂比SEB加Mls刺激的有丝分裂总和增强了三倍。这些结果表明，SEB可能是进一步探索Mls反应的有用工具。
