Aslam Momna, Feleder Carlos, Newsom Ryan J, Campeau Serge, Musteata Florin Marcel
Department of Pharmaceutical Sciences, Albany College of Pharmacy & Health Sciences, 106 New Scotland Avenue, Albany, NY 12208, USA.
Departamento de Investigacion, Instituto de Salud Publica y Efectividad Clinica, Universidad de Ciencias Empresariales y Sociales, C1061ABA CABA, Buenos Aires, Argentina.
Bioanalysis. 2019 Aug;11(16):1523-1534. doi: 10.4155/bio-2019-0144. Epub 2019 Sep 5.
Solid-phase microextraction is proposed to measure concentrations of anandamide and 2-arachidonoyl glycerol in live rat brains in response to stress. Solid-phase microextraction fibers were prepared from steel with 1.5 mm extraction coating. 24 male rats were divided into groups based on brain region, stria terminalis or posterior hypothalamus and loud noise or control groups. The fibers were desorbed in acetonitrile-water (75:25) and analyzed by ultraperformance LC-MS/MS. The linear range of the method was 0.05-50 ng/ml and the concentrations were found to be between 0.3 and 40 ng/ml. The new approach was successfully used to determine the concentrations of anandamide and 2-arachidonoyl glycerol and could be used in the future to measure other endogenous compounds.
固相微萃取法被用于测定应激状态下活大鼠脑内花生四烯酸乙醇胺和2-花生四烯酸甘油酯的浓度。固相微萃取纤维由带有1.5毫米萃取涂层的钢制成。24只雄性大鼠根据脑区(终纹床核或下丘脑后部)以及是否处于噪音环境分为噪音组或对照组。纤维在乙腈-水(75:25)中解吸,并用超高效液相色谱-串联质谱法进行分析。该方法的线性范围为0.05-50纳克/毫升,所测浓度在0.3至40纳克/毫升之间。这种新方法成功用于测定花生四烯酸乙醇胺和2-花生四烯酸甘油酯的浓度,未来可用于测量其他内源性化合物。