School of Chemistry , Sun Yat-sen University , Guangzhou , Guangdong 510275 , People's Republic of China.
Department of Radiology, Sun Yat-sen Memorial Hospital , Sun Yat-sen University , Guangzhou , Guangdong 510120 , People's Republic of China.
J Agric Food Chem. 2019 Feb 27;67(8):2120-2126. doi: 10.1021/acs.jafc.8b06981. Epub 2019 Feb 14.
Endogenous substances, naturally occurring in living organisms, are critical components with physiological and biological functions. Discovery and quantitative measurement of endogenous substances in living biotas are important for food analysis, crop cultivation, and quality assessment. Low or non-invasive in vivo sampling techniques offer the advantages of minimal perturbation to the investigated system and potentially obtain more accurate feedback compared to in vitro sampling. In this perspective, we summarize the up-to-date progress in the development of microdialysis and solid-phase microextraction as valuable tools for in vivo sampling of endogenous substances in food and agriculture chemistry. We discuss their feasibility for on-site and real-time in vivo monitoring and highlight the prospects in searching for highly specific coatings, miniaturized sampling devices, and instruments that well meet the trend for high-efficient and high-throughput analyses.
内源性物质是在生物体内自然产生的，是具有生理和生物学功能的关键组成部分。发现和定量测量生物体内的内源性物质对于食品分析、作物种植和质量评估非常重要。与体外采样相比，低侵入性或非侵入性的体内采样技术具有对被研究系统干扰最小的优点，并且有可能获得更准确的反馈。从这个角度来看，我们总结了微透析和固相微萃取作为食品和农业化学中内源性物质体内采样的有价值工具的最新进展。我们讨论了它们在现场和实时体内监测中的可行性，并强调了在寻找高度特异性涂层、小型化采样设备和仪器方面的前景，这些设备和仪器很好地满足了高效和高通量分析的趋势。
