Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, P. R. China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Biotechnol J. 2020 Feb;15(2):e1900191. doi: 10.1002/biot.201900191. Epub 2019 Sep 17.
Scyllo-inositol (SI), a stereoisomer of inositol, is regarded as a promising therapeutic agent for Alzheimer's disease. Here, an in vitro cofactor-balance biotransformation for the production of SI from myo-inositol (MI) by thermophilic myo-inositol 2-dehydrogenase (IDH) and scyllo-inositol 2-dehydrogenase (SIDH) is presented. These two enzymes (i.e., IDH and SIDH from Geobacillus kaustophilus) are co-expressed in Escherichia coli BL21(DE3), and E. coli cells containing the two enzymes are permeabilized by heat treatment as whole-cell catalysts to convert MI to SI. After condition optimizations about permeabilized temperature, reaction temperature, and initial MI concentration, about 82 g L of SI is produced from 250 g L of MI within 24 h without any cofactor supplementation. This final titer of SI produced is the highest to the authors' limited knowledge. This study provides a promising method for the large-scale industrial production of SI.
船形环氧己醇（SI）是肌醇的立体异构体，被认为是治疗阿尔茨海默病的有前途的治疗剂。在这里，介绍了一种体外辅助因子平衡生物转化方法，用于通过嗜热肌醇 2-脱氢酶（IDH）和船形环氧己醇 2-脱氢酶（SIDH）从肌醇（MI）生产 SI。这两种酶（即来自 Geobacillus kaustophilus 的 IDH 和 SIDH）在大肠杆菌 BL21（DE3）中共同表达，并且含有这两种酶的大肠杆菌细胞通过热处理作为全细胞催化剂进行透化，以将 MI 转化为 SI。在优化透化温度、反应温度和初始 MI 浓度的条件后，在 24 小时内，无需任何辅助因子补充，从 250 g/L 的 MI 中生产出约 82 g/L 的 SI。就作者所知，这是最终 SI 产量的最高水平。这项研究为大规模工业生产 SI 提供了一种很有前途的方法。
