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利用工程化全细胞生物催化剂从（）-（+）-柠檬烯高效合成（）-（+）-紫苏醇

Efficient Synthesis of ()-(+)-Perillyl Alcohol From ()-(+)-Limonene Using Engineered Whole Cell Biocatalyst.

作者信息

Sun Chao, Zhang Rubing, Xie Congxia

机构信息

A State Key Laboratory Base of Eco-Chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, China.

CAS Key Laboratory of Bio-Based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.

出版信息

Front Bioeng Biotechnol. 2022 Apr 25;10:900800. doi: 10.3389/fbioe.2022.900800. eCollection 2022.

DOI:10.3389/fbioe.2022.900800
PMID:35547170
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9084310/
Abstract

()-(+)-perillyl alcohol is a much valued supplemental compound with a wide range of agricultural and pharmacological characteristics. The aim of this study was to improve ()-(+)-perillyl alcohol production using a whole-cell catalytic formula. In this study, we employed plasmids with varying copy numbers to identify an appropriate strain, strain 03. We demonstrated that low levels of alKL provided maximal biocatalyst stability. Upon determination of the optimal conditions, the ()-(+)-perillyl alcohol yield reached 130 mg/L. For cofactor regeneration, we constructed strain 10, expressing FDH from , and achieved ()-(+)-perillyl alcohol production of 230 mg/L. As a result, 1.23 g/L ()-(+)-perillyl alcohol was transformed in a 5 L fermenter. Our proposed method facilitates an alternative approach to the economical biosynthesis of ()-(+)-perillyl alcohol.

摘要

（）-（+）-紫苏醇是一种具有广泛农业和药理特性的重要补充化合物。本研究的目的是使用全细胞催化配方提高（）-（+）-紫苏醇的产量。在本研究中，我们使用了不同拷贝数的质粒来鉴定合适的菌株，即菌株03。我们证明低水平的alKL可提供最大的生物催化剂稳定性。确定最佳条件后，（）-（+）-紫苏醇产量达到130mg/L。为了进行辅因子再生，我们构建了表达来自[具体来源未给出]的FDH的菌株10，并实现了230mg/L的（）-（+）-紫苏醇产量。结果，在5L发酵罐中转化得到了1.23g/L的（）-（+）-紫苏醇。我们提出的方法为（）-（+）-紫苏醇的经济生物合成提供了一种替代方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6584/9084310/0a8e961a2332/fbioe-10-900800-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6584/9084310/b73d163d531c/fbioe-10-900800-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6584/9084310/dee640c5fb88/fbioe-10-900800-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6584/9084310/11d83f78fb9e/fbioe-10-900800-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6584/9084310/5e20e34466e2/fbioe-10-900800-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6584/9084310/0a8e961a2332/fbioe-10-900800-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6584/9084310/b73d163d531c/fbioe-10-900800-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6584/9084310/dee640c5fb88/fbioe-10-900800-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6584/9084310/11d83f78fb9e/fbioe-10-900800-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6584/9084310/5e20e34466e2/fbioe-10-900800-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6584/9084310/0a8e961a2332/fbioe-10-900800-g005.jpg

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