State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Microb Pathog. 2019 Dec;137:103705. doi: 10.1016/j.micpath.2019.103705. Epub 2019 Sep 2.
Nocardia farcinica is the etiological agent of nocardiosis, leading to serious pulmonary or systemic infections. To uncover virulence factors and early diagnostic markers, secreted proteins of N. farcinica IFM 10152 were analyzed using an immunoproteome-based approach. A total of 5 proteins were identified by matrix-assisted laser desorption (MALDI-TOF-MS). Bioinformatic analyses showed that the identified proteins were involved in defense against the host innate immune system and required for pathogenesis. All proteins were expressed in E. coli and antigenicity was analyzed with Western blot. To our knowledge, these proteins with antigenicity were identified for the first time in N. farcinica and they may help elucidate the pathogenesis underlying Nocardia and provide potential future diagnostic markers.
星形诺卡氏菌是诺卡氏菌病的病原体，可导致严重的肺部或全身性感染。为了揭示毒力因子和早期诊断标志物，采用基于免疫蛋白质组学的方法分析了星形诺卡氏菌 IFM 10152 的分泌蛋白。通过基质辅助激光解吸（MALDI-TOF-MS）鉴定了总共 5 种蛋白质。生物信息学分析表明，鉴定出的蛋白质参与宿主固有免疫系统的防御，并且是发病所必需的。所有蛋白质均在大肠杆菌中表达，并通过 Western blot 分析其抗原性。据我们所知，这些具有抗原性的蛋白质在星形诺卡氏菌中是首次被鉴定出来的，它们可能有助于阐明星形诺卡氏菌的发病机制，并为未来提供潜在的诊断标志物。
