Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Department of Nutrition and Food Hygiene, School of Health Science, Wuhan University, 185, Donghu Rd, Wuhan, Hubei 430071, China.
Pregnancy Hypertens. 2019 Jul;17:216-225. doi: 10.1016/j.preghy.2019.06.009. Epub 2019 Jun 29.
To identify circRNA expression profiles in the placentae of severe preeclampsia (SPE) and normal pregnant (NP) women.
Placental samples were collected from six paired SPE and NP women. CircRNA expression profiles were identified by RNA-Seq and validated in another 30 SPE and NP samples by qRT-PCR. Several bioinformatic tools were utilised to analyse the potential function of differentially expressed circRNAs (DE-circRNAs) and to predict target microRNAs (miRNAs) and proteins. Furthermore, immunohistochemistry, Western blotting and RNA binding protein immunoprecipitation (RIP) were conducted to confirm the interaction between the circRNA and protein.
In total, 18,631 circRNAs were detected. Among them, 180 circRNAs were differentially expressed, including 94 upregulated and 86 downregulated circRNAs. Seven DE-circRNAs were selected for validation, and the results of six circRNAs (hsa_circ_0007611, hsa_circ_0011460, hsa_circ_0002888, and hsa_circ_0007445, hsa_circ_0017068, hsa_circ_0012737) were consistent with the sequencing results. Bioinformatics analyses of DE-circRNAs revealed that most of them are involved in vasodilation, regulation of blood vessel size, protein transport and localization, and pathways in cancer. In addition to the mRNA expression profile, it was interesting to find that the hsa_circ_0011460 target gene solute carrier organic anion transporter family member 2A1 (SLCO2A1, PGT) was also significantly increased in SPE placentae. Immunohistochemistry, Western blotting and qRT-PCR validated the expression and distribution of PGT. Finally, RIP of HTR-8/SVneo cells confirmed that hsa_circ_0011460 targets PGT directly.
A total of 180 DE-circRNAs were detected. One crucial circRNA, hsa_circ_0011460, was shown to interact directly with its host gene PGT. These findings indicated that hsa_circ_0011460 may serve as a potential therapeutic target for patients with SPE.
鉴定严重先兆子痫（SPE）和正常妊娠（NP）妇女胎盘中环状 RNA 表达谱。
收集六对 SPE 和 NP 妇女的胎盘样本。通过 RNA-Seq 鉴定环状 RNA 表达谱，并通过 qRT-PCR 在另外 30 个 SPE 和 NP 样本中进行验证。利用几种生物信息学工具分析差异表达的环状 RNA（DE-circRNA）的潜在功能，并预测靶 microRNA（miRNA）和蛋白质。此外，进行免疫组织化学、Western blot 和 RNA 结合蛋白免疫沉淀（RIP）实验以确认 circRNA 和蛋白质之间的相互作用。
共检测到 18631 个 circRNA。其中，有 180 个 circRNA 差异表达，包括 94 个上调和 86 个下调的 circRNA。选择了 7 个 DE-circRNA 进行验证，其中 6 个 circRNA（hsa_circ_0007611、hsa_circ_0011460、hsa_circ_0002888 和 hsa_circ_0007445、hsa_circ_0017068、hsa_circ_0012737）的结果与测序结果一致。DE-circRNA 的生物信息学分析表明，它们大多数参与血管舒张、血管大小调节、蛋白质运输和定位以及癌症途径。除了 mRNA 表达谱外，有趣的是发现 hsa_circ_0011460 的靶基因溶质载体有机阴离子转运家族成员 2A1（SLCO2A1，PGT）在 SPE 胎盘中也显著增加。免疫组织化学、Western blot 和 qRT-PCR 验证了 PGT 的表达和分布。最后，HTR-8/SVneo 细胞的 RIP 证实 hsa_circ_0011460 直接靶向 PGT。
共检测到 180 个 DE-circRNA。一个关键的 circRNA，hsa_circ_0011460，被证明与它的宿主基因 PGT 直接相互作用。这些发现表明 hsa_circ_0011460 可能作为 SPE 患者的潜在治疗靶点。
