膜联蛋白A1对口腔鳞状细胞癌中TPF化疗敏感性的影响及机制
[The effect and mechanism of ANXA1 on TPF chemosensitivity in oral squamous cell carcinoma].
作者信息
Zhu Dong-Wang, Sun Wen-Wen, Zhao Tong-Chao, Zhong Lai-Ping, Zhang Zhi-Yuan
机构信息
Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology. Shanghai 200011, China. E-mail:
出版信息
Shanghai Kou Qiang Yi Xue. 2019 Jun;28(3):225-230.
PURPOSE
To investigate the mechanism of ANXA1 in TPF chemotherapy of oral squamous cell carcinoma (OSCC).
METHODS
ANXA1 overexpression and low-expression cell lines were constructed. The role of ANXA1 in TPF chemotherapy was analyzed by cell proliferation, cytotoxicity test, real-time PCR and Western blot, and the mechanism of ANXA1 in TPF chemotherapy through EMT (epithelial-mesenchymal transition) pathway was discussed. The data were analyzed with SPSS 18.0 software package.
RESULTS
After overexpression of ANXA1, cell growth rate decreased, cell cycle slowed down, sensitivity to TPF-induced drugs decreased, and EMT occurred in OSCC. After underexpression of ANXA1, cell growth rate increased, cell cycle accelerated, sensitivity to TPF chemotherapeutic drugs increased, and reverse EMT occurred in OSCC.
CONCLUSIONS
In TPF chemotherapy of OSCC, overexpression of ANXA1 results in EMT of cells, which leads to decreased chemosensitivity.
目的
探讨膜联蛋白A1（ANXA1）在口腔鳞状细胞癌（OSCC）TPF化疗中的作用机制。
方法
构建ANXA1过表达和低表达细胞系。通过细胞增殖、细胞毒性试验、实时定量聚合酶链反应（PCR）和蛋白质免疫印迹法分析ANXA1在TPF化疗中的作用，并探讨ANXA1通过上皮-间质转化（EMT）途径在TPF化疗中的机制。采用SPSS 18.0软件包进行数据分析。
结果
ANXA1过表达后，细胞生长速率降低，细胞周期减慢，对TPF诱导药物的敏感性降低，且OSCC发生EMT。ANXA1低表达后，细胞生长速率增加，细胞周期加速，对TPF化疗药物的敏感性增加，且OSCC发生EMT逆转。
结论
在OSCC的TPF化疗中，ANXA1过表达导致细胞发生EMT，进而导致化疗敏感性降低。
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