Skak-Nielsen T, Holst J J, Christensen J D, Fjalland B
Institute of Biology, Royal Danish School of Pharmacy, Copenhagen.
Regul Pept. 1988 Oct;23(1):95-104. doi: 10.1016/0167-0115(88)90425-9.
We studied the effects of the neuropeptide gastrin-releasing peptide on pepsinogen secretion using an isolated perfused rat stomach with intact vagal innervation. Following electrical stimulation of the vagus nerves, the pepsin output to the luminal effluent increased from 94 +/- 7 to 182 +/- 24 units pepsin/min and the release of immunoreactive gastrin-releasing peptide to the venous effluent increased from 0.059 +/- 0.014 to 0.138 +/- 0.028 pmol/min. Infusion of gastrin-releasing peptide at 10(-8) M significantly increased pepsin output (from 87 +/- 17 to 129 +/- 22 units pepsin/min) and simultaneous infusion of gastrin-releasing peptide and carbachol at 10(-8) and 10(-6) M, respectively, resulted in an increase to almost 4 times the basal values. Atropine reduced but did not abolish the pepsin response to vagal stimulation and to infusion of gastrin-releasing peptide. Our results suggest that gastrin-releasing peptide participates in the vagal control of pepsinogen secretion.
我们使用具有完整迷走神经支配的离体灌注大鼠胃，研究了神经肽胃泌素释放肽对胃蛋白酶原分泌的影响。迷走神经电刺激后，向腔内流出液中的胃蛋白酶输出量从94±7单位胃蛋白酶/分钟增加到182±24单位胃蛋白酶/分钟，向静脉流出液中免疫反应性胃泌素释放肽的释放量从0.059±0.014皮摩尔/分钟增加到0.138±0.028皮摩尔/分钟。以10⁻⁸ M的浓度输注胃泌素释放肽显著增加了胃蛋白酶输出量（从87±17单位胃蛋白酶/分钟增加到129±22单位胃蛋白酶/分钟），同时分别以10⁻⁸ M和10⁻⁶ M的浓度输注胃泌素释放肽和卡巴胆碱，导致胃蛋白酶输出量增加到几乎是基础值的4倍。阿托品降低了但并未消除对迷走神经刺激和输注胃泌素释放肽的胃蛋白酶反应。我们的结果表明，胃泌素释放肽参与了迷走神经对胃蛋白酶原分泌的控制。
