Blackburn M J, Patt H M
Exp Hematol. 1979 Jul;7(6):324-7.
Media conditioned by marrow adherent cells from anaemic Sl/Sld and W/Wv mice increased the 24-h survival of CBA CFUs in vitro compared to fresh medium to about the same extent as marrow-conditioned medium from normal Sl+/Sl+, W+/W+, and CBA mice. Sl/Sld marrow-conditioned medium also increased the percentage of CFUs in DNA synthesis to the same extent as CBA marrow-conditioned medium. These results demonstrate that Sl/Sld mice produce a marrow stromal factor that increases both survival of CFUs and the percentage of CFUs in DNA synthesis in vitro. Therefore, the defective haemopoietic microenvironment of Sl/Sld mice is not due to a deficiency in the production of this factor.
与新鲜培养基相比，来自贫血的Sl/Sld和W/Wv小鼠的骨髓贴壁细胞条件培养基在体外使CBA集落形成单位（CFU）的24小时存活率提高，提高程度与来自正常Sl+/Sl+、W+/W+和CBA小鼠的骨髓条件培养基大致相同。Sl/Sld骨髓条件培养基还使处于DNA合成期的CFU百分比增加，增加程度与CBA骨髓条件培养基相同。这些结果表明，Sl/Sld小鼠产生一种骨髓基质因子，该因子在体外既能提高CFU的存活率，又能提高处于DNA合成期的CFU百分比。因此，Sl/Sld小鼠造血微环境缺陷并非由于该因子产生不足所致。
