State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China.
J Nanosci Nanotechnol. 2020 Apr 1;20(4):2138-2143. doi: 10.1166/jnn.2020.17327.
With the higher and higher application level of medical technology, more and more genetic diseases have been diagnosed. Nucleic acid, as an important genetic material, has been found to have important functions in the storage and transmission of the genetic information in the replication and synthesis of proteins. As the first step in nucleic acid detection experiments, nucleic acid extraction performance is associated with the purity of target nucleic acid samples, which is very important for the downstream steps. In this paper, we employed the magnetic bead for extracting nucleic acids based on the platform of large liquid handling workstation and designed a matching magnetic separation module. It was shown that the temperature control block designed in this paper has reliable stability, high accuracy by using the incremental PID algorithm, with the control accuracy up to ±0.5 °C, and the control stabilization time is about 90 s, which can satisfy the experimental requirements. Besides, the average magnetic bead transfer rate of this module was further verified by mimicking the manual magnetic bead nucleic acid extraction process. The results proved that the module has an excellent performance with the average magnetic bead transfer rate greater than 95% and the magnetic bead transfer rate in each well greater than 90%, which could be consistent with the experimental indictors of nucleic acid extraction.
随着医学技术应用水平的不断提高，越来越多的遗传性疾病得到了诊断。核酸作为一种重要的遗传物质，在遗传信息的储存和传递、蛋白质的复制和合成中都具有重要的功能。作为核酸检测实验的第一步，核酸提取性能与目标核酸样品的纯度有关，这对下游步骤非常重要。本文基于大型液体处理工作站平台，采用磁珠法提取核酸，并设计了匹配的磁分离模块。结果表明，本文设计的控温块采用增量 PID 算法，具有可靠的稳定性和高精度，控制精度可达±0.5°C，控制稳定时间约为 90s，能够满足实验要求。此外，通过模拟手动磁珠核酸提取过程，进一步验证了该模块的平均磁珠转移率。结果表明，该模块具有优异的性能，平均磁珠转移率大于 95%，每个孔的磁珠转移率大于 90%，与核酸提取的实验指标一致。
