School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
J Nanosci Nanotechnol. 2020 Mar 1;20(3):1678-1684. doi: 10.1166/jnn.2020.16517.
Green synthesis offers an eco-friendly and low-cost approach for the synthesis of silver nanoparticles (AgNPs). Many studies have reported on the use of biomolecules, especially plant extracts, as reducing and/or stabilizing agents in place of toxic chemicals. This study reports on the use of bacterial genomic DNA as an alternative stabilizing agent for the green synthesis of AgNPs under light activation. With both increased DNA quantities and reaction times under light exposure, more stabilized AgNPs formed as indicated by the surface plasmon resonance intensities. The synthesized AgNPs were spherical with an average size of 61.36±10.15 nm as calculated using the dynamic light scattering (DLS) technique. The X-ray diffraction, selected area electron diffraction, and high resolution transmission electron microscope (TEM) analyses confirmed the formation of face-centered cubic (fcc) structured AgNPs. The produced AgNPs exhibited antibacterial activities against both Gram-negative and Gram-positive , suggesting its potential application as an antibacterial agent.
绿色合成提供了一种环保且低成本的方法来合成银纳米粒子（AgNPs）。许多研究报告都使用生物分子，特别是植物提取物，作为替代有毒化学物质的还原剂和/或稳定剂。本研究报告了在光激活下使用细菌基因组 DNA 作为绿色合成 AgNPs 的替代稳定剂。随着 DNA 数量的增加和在光暴露下的反应时间的增加，形成了更多稳定的 AgNPs，这可以通过表面等离子体共振强度来指示。使用动态光散射（DLS）技术计算得到，所合成的 AgNPs 呈球形，平均粒径为 61.36±10.15nm。X 射线衍射、选区电子衍射和高分辨率透射电子显微镜（TEM）分析证实了面心立方（fcc）结构 AgNPs 的形成。所制备的 AgNPs 对革兰氏阴性菌和革兰氏阳性菌均表现出抗菌活性，表明其作为抗菌剂的潜在应用。
