基于荧光噬菌体展示技术的B细胞筛选高通量平台

High-Throughput Platform for B-Cell Screening Based on Fluorescent Phage-Display Technology.

作者信息

Lomakin Ya A, Kaminskaya A N, Stepanov A V, Shmidt A A, Gabibov A G, Belogurov A A

机构信息

M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

Kazan (Volga region) Federal University, Kazan, Republic of Tatarstan, Russia.

出版信息

Bull Exp Biol Med. 2019 Aug;167(4):446-451. doi: 10.1007/s10517-019-04546-5. Epub 2019 Sep 6.

DOI:10.1007/s10517-019-04546-5

PMID:31493253

Abstract

A system for detection of malignantly transformed cells, including follicular lymphoma Bcells, was developed and experimentally validated. The system is based on the use of bacteriophages carrying exposed ligands for pathogenic B-cell receptors. The efficiency of binding to target cells is several times higher than in systems with chemically synthesized biotinylated peptides. The new method is proposed as a noninvasive diagnostic test for mapping B-cell lymphoma and for determining the specificity of B-cell receptors and high-throughput combinatorial selection of various repertories of B cells.

摘要

开发了一种用于检测恶性转化细胞（包括滤泡性淋巴瘤B细胞）的系统，并进行了实验验证。该系统基于使用携带针对致病性B细胞受体的暴露配体的噬菌体。与化学合成的生物素化肽系统相比，其与靶细胞的结合效率高出数倍。该新方法被提议作为一种非侵入性诊断测试，用于绘制B细胞淋巴瘤图谱、确定B细胞受体的特异性以及对各种B细胞库进行高通量组合选择。