原核细胞和真核细胞对玉米赤霉烯酮的生物吸附和代谢研究。
A study of zearalenone biosorption and metabolisation by prokaryotic and eukaryotic cells.
机构信息
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarina 7 St, PL-87-100 Toruń, Poland; Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, Wileńska 4, 87-100 Torun, Poland.
Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, Wileńska 4, 87-100 Torun, Poland.
出版信息
Toxicon. 2019 Nov;169:81-90. doi: 10.1016/j.toxicon.2019.09.008. Epub 2019 Sep 4.
A study of the mechanism responsible for the zearalenone (ZEA) neutralization by lactic acid bacteria Lactococcus lactis 56 and L929 cell line was carried out by determination of the kinetics of the binding process. In the case of prokaryotic cells the biosorption process was non-linear and three steps were identified. The maximum efficiency of zearalenone binding to L. lactis was almost 30% and no metabolites were observed. In turn, for eukaryotic cells only two steps of the binding process were differentiated, and the efficiency of zearalenone binding was 53.99%. Furthermore, L929 cell line metabolizes zearalenone to α-ZOL and β-ZOL. Additionally, Fourier transform infrared spectroscopy (FTIR) was used for description of the structural changes at the protein and lipid level, while Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS) was applied to detect changes at the molecular level.
本研究旨在通过测定结合过程的动力学，研究乳酸菌 Lactococcus lactis 56 和 L929 细胞系对玉米赤霉烯酮（ZEA）的中和作用机制。在原核细胞中，生物吸附过程是非线性的，并确定了三个步骤。L. lactis 对玉米赤霉烯酮的最大结合效率接近 30%，且未观察到代谢产物。相反，对于真核细胞，仅区分了结合过程的两个步骤，玉米赤霉烯酮的结合效率为 53.99%。此外，L929 细胞系将玉米赤霉烯酮代谢为α-ZOL 和 β-ZOL。此外，傅里叶变换红外光谱（FTIR）用于描述蛋白质和脂质水平的结构变化，而基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）用于检测分子水平的变化。
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