Department of Anatomy and Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
Department of Anatomy and Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan; Department of Medical Science and Technology, Faculty of Health Sciences, Hiroshima International University, 555-36 Kurosegakuendai, Higashihiroshima City, Hiroshima 739-2695, Japan.
Mech Dev. 2019 Oct;159:103568. doi: 10.1016/j.mod.2019.103568. Epub 2019 Sep 4.
The axial skeleton is divided into different regions based on its morphological features. In particular, in birds and mammals, ribs are present only in the thoracic region. The axial skeleton is derived from a series of somites. In the thoracic region of the axial skeleton, descendants of somites coherently penetrate into the somatic mesoderm to form ribs. In regions other than the thoracic, descendants of somites do not penetrate the somatic lateral plate mesoderm. We performed live-cell time-lapse imaging to investigate the difference in the migration of a somite cell after contact with the somatic lateral plate mesoderm obtained from different regions of anterior-posterior axis in vitro on cytophilic narrow paths. We found that a thoracic somite cell continues to migrate after contact with the thoracic somatic lateral plate mesoderm, whereas it ceases migration after contact with the lumbar somatic lateral plate mesoderm. This suggests that cell-cell interaction works as an important guidance cue that regulates migration of somite cells. We surmise that the thoracic somatic lateral plate mesoderm exhibits region-specific competence to allow penetration of somite cells, whereas the lumbosacral somatic lateral plate mesoderm repels somite cells by contact inhibition of locomotion. The differences in the behavior of the somatic lateral plate mesoderm toward somite cells may confirm the distinction between different regions of the axial skeleton.
轴骨骼根据其形态特征分为不同区域。特别是在鸟类和哺乳动物中，肋骨仅存在于胸部区域。轴骨骼源自一系列体节。在轴骨骼的胸部区域，体节的后代一致地穿透体壁中胚层形成肋骨。在胸部以外的区域，体节的后代不会穿透体壁侧板中胚层。我们进行了活细胞延时成像，以研究在体外沿亲代细胞狭窄路径接触来自前后轴不同区域的体壁侧板时，体节细胞迁移的差异。我们发现，与胸体壁侧板接触后，胸体节细胞继续迁移，而与腰体壁侧板接触后，其迁移停止。这表明细胞-细胞相互作用作为调节体节细胞迁移的重要导向线索。我们推测，胸体壁侧板表现出区域特异性能力，允许体节细胞穿透，而腰荐体壁侧板通过运动接触抑制排斥体节细胞。体壁侧板对体节细胞的行为差异可能证实了轴骨骼不同区域的区分。
