School of Resource and Environment, Northeast Agricultural University, Harbin, 150030, China.
School of Resource and Environment, Northeast Agricultural University, Harbin, 150030, China.
Ecotoxicol Environ Saf. 2019 Nov 30;184:109616. doi: 10.1016/j.ecoenv.2019.109616. Epub 2019 Sep 4.
The application of biogas slurry as an organic fertilizer is a promising method for utilizing breeding manure wastewater. At present, the impact of biogas slurry on the properties of organic matter in soil is not clear. In this study, a pot experiment in which chemical fertilizers were replaced with biogas slurry from a swine farm was performed. The fluorescence spectra combined with parallel factor (PARAFAC) analysis and principal component analysis (PCA) were used to explore the influence of biogas slurry on the protein and humic substance contents in the dissolved organic matter (DOM) in soil. The results showed that there were two proteins (component 3 (C3) and component 4 (C4)) and two humic substances ( component 1 (C1) and component 2 (C2)) in the DOM of the experimental soil. The application of swine biogas slurry can significantly increase the content of DOM in soil, but the increase was weakened with extended time. Compared with the CKA, the biogas slurry significantly increased the C1, C2, C3 and C4 contents in the initial stage by 116.17%, 76.41%, 578.71% and 278.13%, respectively. Within 28 days of planting corn, proteins with simple molecular structure in the DOM in the soil began to be transformed into humic substances with high molecular weight and more complex molecular structures. On the 60th day, the contents of C1 and C2 in the DOM of the treated treatments soil increased by 13.72%-34.40% and 5.05%-17.78% respectively, and tyrosine content decreased by 90.11%-94.41%. This study provides a new perspective on the effects of biogas slurry application on soil properties and sustainable utilization of soil.
沼气液作为有机肥的应用是利用养殖粪污的一种有前景的方法。目前，沼气液对土壤中有机质性质的影响尚不清楚。本研究进行了以沼气液替代猪场化肥的盆栽实验，采用荧光光谱结合平行因子（PARAFAC）分析和主成分分析（PCA），探讨了沼气液对土壤中溶解性有机质（DOM）中蛋白质和腐殖质含量的影响。结果表明，实验土壤 DOM 中存在两种蛋白质（组分 3（C3）和组分 4（C4））和两种腐殖质（组分 1（C1）和组分 2（C2））。施用猪沼气液能显著增加土壤中 DOM 的含量，但随时间延长增加趋势减弱。与 CK-A 相比，沼气液在初期分别显著增加了 DOM 中 C1、C2、C3 和 C4 的含量，增幅分别为 116.17%、76.41%、578.71%和 278.13%。在玉米种植的 28 天内，土壤 DOM 中结构简单的蛋白质开始转化为高分子量、结构更复杂的腐殖质。到第 60 天，处理组土壤 DOM 中 C1 和 C2 的含量分别增加了 13.72%-34.40%和 5.05%-17.78%，酪氨酸含量下降了 90.11%-94.41%。本研究为沼气液应用对土壤性质和土壤可持续利用的影响提供了新视角。
