College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, Hubei, PR China; Aquatic Vegetable Preservation and Processing Technology Engineering Centre of Hubei Province, Wuhan 430070, Hubei, PR China.
Food Chem. 2020 Feb 1;305:125435. doi: 10.1016/j.foodchem.2019.125435. Epub 2019 Aug 29.
Soluble dietary fibre (SDF) of micronized and non-micronized powders of lotus root nodes were investigated based on its adsorption and activity inhibition of pancreatic lipase (PL) by using circular dichroism, fluorescence spectroscopy and modification. Results showed that SDF2 (SDF from micronized powders of lotus root nodes) had stronger PL adsorption and enzyme activity inhibition than SDF1 (SDF from non-micronized powders of lotus root nodes). Specifically, SDF2 showed more binding sites than SDF1 in PL. There were hydrogen bonds and van der Waals interactions between SDF and PL, with Trp on PL probably serving as the main binding site. Carboxyl groups exhibited a stronger inhibition on PL by carboxymethyl and hydroxypropyl modification. The common mechanisms between SDF1 and SDF2 can be attributed to the combination between Trp and carboxyl groups, while the differences may be generated by the variations in structures or chemical groups induced by micronization.
研究了微细化和未微细化莲藕节点粉末的可溶性膳食纤维（SDF），通过圆二色性、荧光光谱和修饰来研究其对胰脂肪酶（PL）的吸附和活性抑制作用。结果表明，SDF2（来自微细化莲藕节点粉末的 SDF）比 SDF1（来自未微细化莲藕节点粉末的 SDF）具有更强的 PL 吸附和酶活性抑制作用。具体来说，SDF2 在 PL 中显示出比 SDF1 更多的结合位点。SDF 和 PL 之间存在氢键和范德华相互作用，PL 上的色氨酸可能是主要的结合位点。羧甲基和羟丙基修饰后，羧基对 PL 的抑制作用更强。SDF1 和 SDF2 之间的共同机制可归因于色氨酸和羧基之间的结合，而差异可能是由微细化引起的结构或化学基团的变化产生的。
